Thermal Outlining using Focused Ultrasound (TOFU) with reversible temperature sensitive fluorescent probes

Tiffany C. Kwong; Farouk Nouizi; Yuting Lin; Yue Zhu; Uma Sampathkumaran; Gultekin Gulsen

doi:10.1117/12.2213799

10 March 2016 Thermal Outlining using Focused Ultrasound (TOFU) with reversible temperature sensitive fluorescent probes

Tiffany C. Kwong, Farouk Nouizi, Yuting Lin, Yue Zhu, Uma Sampathkumaran, Gultekin Gulsen

Author Affiliations +

Proceedings Volume 9701, Multimodal Biomedical Imaging XI; 97010L (2016) https://doi.org/10.1117/12.2213799
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Optical imaging has long been hindered by the high absorption and scattering of light in biological tissue. This makes it difficult to probe beyond a few millimeters beneath the surface without sacrificing image resolution and quantitative accuracy. Strong scattering and the inherent nature of the inverse problem makes fluorescence diffuse optical tomography (FT) extremely challenging. To this end, multi-modality techniques that combine anatomical imaging with the functional optical information have been used to improve the resolution and accuracy of FT. Previously, we have reported on the feasibility of a new imaging method, "Thermal Outlining using Focused Ultrasound" (TOFU), which combines the sensitivity of FT with the resolution of focused ultrasound using temperature reversible fluorescent probes. In this method, the position of the temperature reversible fluorescent probes is localized by an increase in fluorescent signal when the hot spot of the focused ultrasound beam is scanned over the medium. This a priori information is then utilized to guide and constrain conventional reconstruction algorithm to recover the position and concentration of the probes more accurately. The small size of the focal spot (~1.4 mm) up to a depth of 6 cm, allows imaging the distribution of these temperature sensitive agents with not only high spatial resolution but also high quantitative accuracy in deep tissue. In this work, the performance of the system will be evaluated using simulation and phantoms to investigate the dependence that size of the fluorescent distribution has on the TOFU system performance.

Conference Presentation

Citation Download Citation

Tiffany C. Kwong, Farouk Nouizi, Yuting Lin, Yue Zhu, Uma Sampathkumaran, and Gultekin Gulsen "Thermal Outlining using Focused Ultrasound (TOFU) with reversible temperature sensitive fluorescent probes", Proc. SPIE 9701, Multimodal Biomedical Imaging XI, 97010L (10 March 2016); https://doi.org/10.1117/12.2213799

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available