Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging

Lixin Liu; Artem Pliss; Xiao Peng; Andrey Kuzmin; Junle Qu; Paras N. Prasad

doi:10.1117/12.2211889

14 March 2016 Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging

Lixin Liu, Artem Pliss, Xiao Peng, Andrey Kuzmin, Junle Qu, Paras N. Prasad

Author Affiliations +

Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 971221 (2016) https://doi.org/10.1117/12.2211889
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Measurements and monitoring of concentrations of macromolecules in live cells and sub-cellular structures is of tremendous interest in cell biology and translational medicine. In this report we demonstrate a breakthrough potential of FLIM for real-time quantitative mapping of macromolecular distribution in the cell. In our approach we exploit a correlation existing between the fluorescence lifetime of fluorophores, refractive index and local concentrations of cellular macromolecules in the of fluorophore's microenvironment. We show a value of our approach for fundamental cell science and cellular diagnostic assays.

Citation Download Citation

Lixin Liu, Artem Pliss, Xiao Peng, Andrey Kuzmin, Junle Qu, and Paras N. Prasad "Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971221 (14 March 2016); https://doi.org/10.1117/12.2211889

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