Towards field malaria diagnosis using surface enhanced Raman spectroscopy

Keren Chen; Aoli Xiong; Clement Yuen; Peter Preiser; Quan Liu

doi:10.1117/12.2227042

27 April 2016 Towards field malaria diagnosis using surface enhanced Raman spectroscopy

Keren Chen, Aoli Xiong, Clement Yuen, Peter Preiser, Quan Liu

Proceedings Volume 9887, Biophotonics: Photonic Solutions for Better Health Care V; 98870M (2016) https://doi.org/10.1117/12.2227042
Event: SPIE Photonics Europe, 2016, Brussels, Belgium

Abstract

We report three strategies of surface enhanced Raman spectroscopy (SERS) for β-hematin and hemozoin detection in malaria infected human blood, which can be potentially developed for field malaria diagnosis. In the first strategy, we used silver coated magnetic nanoparticles (Fe₃O₄@Ag) in combination with an external magnetic field to enhance the Raman signal of β-hematin. Then we developed two SERS methods without the requirement of magnetic field for malaria infection diagnosis. In Method 1, silver nanoparticles were synthesized separately and then mixed with lysed blood just like in traditional SERS measurements; while in Method 2, we developed an ultrasensitive SERS method by synthesizing silver nanoparticles directly inside the parasites of Plasmodium falciparum. Method 2 can be also used to detect single parasites in the ring stage.

Citation Download Citation

Keren Chen, Aoli Xiong, Clement Yuen, Peter Preiser, and Quan Liu "Towards field malaria diagnosis using surface enhanced Raman spectroscopy", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98870M (27 April 2016); https://doi.org/10.1117/12.2227042

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