The development of attenuation compensation models of fluorescence spectroscopy signals

Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova; Andrey V Dunaev

doi:10.1117/12.2229451

21 April 2016 The development of attenuation compensation models of fluorescence spectroscopy signals

Victor V. Dremin, Evgeny A. Zherebtsov, Ilya E. Rafailov, Andrey Y. Vinokurov, Irina N. Novikova, Angelina I. Zherebtsova, Karina S. Litvinova, Andrey V Dunaev

Author Affiliations +

Proceedings Volume 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS); 99170Y (2016) https://doi.org/10.1117/12.2229451
Event: Saratov Fall Meeting 2015, 2015, Saratov, Russian Federation

Abstract

This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry.

We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra.

In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.

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Victor V. Dremin, Evgeny A. Zherebtsov, Ilya E. Rafailov, Andrey Y. Vinokurov, Irina N. Novikova, Angelina I. Zherebtsova, Karina S. Litvinova, and Andrey V Dunaev "The development of attenuation compensation models of fluorescence spectroscopy signals", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (21 April 2016); https://doi.org/10.1117/12.2229451

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