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We perform micron-level co-registration of two-photon fluorescence microscopy (TPFM) images with en face frozen section analysis (FSA) histology. We demonstrate that TPFM has excellent sensitivity and specificity for evaluating squamous cell carcinoma (SCC) on surgical margins.
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Chi Huang, Vincent D. Ching-Roa, Sherrif F. Ibrahim, Michael G. Giacomelli, "Evaluation of squamous cell carcinoma surgical margins using two-photon fluorescence microscopy," Proc. SPIE PC11934, Photonics in Dermatology and Plastic Surgery 2022, PC119340D (7 March 2022); https://doi.org/10.1117/12.2608414