Nachiket Kulkarnihttps://orcid.org/0000-0002-1780-5388,1 Andrew Masciola,2 Abhinav Nishant,1 Kyungjo Kim,1 Arthur F. Gmitro,2 Esther E. Freeman,3 Aggrey Semeere,4 Miriam Nakalembe,4 DongKyun Kang1
1Wyant College of Optical Sciences (United States) 2The Univ. of Arizona (United States) 3Massachusetts General Hospital (United States) 4Infectious Diseases Institute (Uganda)
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We have developed an axially spectrally-encoded confocal endomicroscope that can visualize multiple depths of the tissue in a single image frame. We developed a custom hyperchromatic objective lens (focal length = 4.8 mm; NA = 0.68) to focus different wavelengths from a broadband light source into different axial depths of the tissue. The confocal endomicroscope achieved lateral and axial resolution of 2 μm and 4 μm, respectively over a lateral field of 468 μm and an axial depth range of 100 μm. The material cost was < $1500. Preliminary confocal images of human tissues in vivo clearly visualized cellular details.
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Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyungjo Kim, Arthur F. Gmitro, Esther E. Freeman, Aggrey Semeere, Miriam Nakalembe, DongKyun Kang, "Axial spectral encoding for low-cost confocal endomicroscopy," Proc. SPIE PC11937, Endoscopic Microscopy XVII, PC119370P (7 March 2022); https://doi.org/10.1117/12.2609006