Presentation
7 March 2022 Axial spectral encoding for low-cost confocal endomicroscopy
Author Affiliations +
Proceedings Volume PC11937, Endoscopic Microscopy XVII; PC119370P (2022) https://doi.org/10.1117/12.2609006
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We have developed an axially spectrally-encoded confocal endomicroscope that can visualize multiple depths of the tissue in a single image frame. We developed a custom hyperchromatic objective lens (focal length = 4.8 mm; NA = 0.68) to focus different wavelengths from a broadband light source into different axial depths of the tissue. The confocal endomicroscope achieved lateral and axial resolution of 2 μm and 4 μm, respectively over a lateral field of 468 μm and an axial depth range of 100 μm. The material cost was < $1500. Preliminary confocal images of human tissues in vivo clearly visualized cellular details.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyungjo Kim, Arthur F. Gmitro, Esther E. Freeman, Aggrey Semeere, Miriam Nakalembe, and DongKyun Kang "Axial spectral encoding for low-cost confocal endomicroscopy", Proc. SPIE PC11937, Endoscopic Microscopy XVII, PC119370P (7 March 2022); https://doi.org/10.1117/12.2609006
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KEYWORDS
Confocal microscopy

Computer programming

Endomicroscopy

Tissue optics

Light sources

Tissues

Objectives

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