SRS imaging of lipid-associated mitochondrial dysfunction in human skeletal muscle

Lingyan Shi; Phyllis Chang; Audrey Zeng; Yajuan Li; Bryan Bergman

doi:10.1117/12.2614093

7 March 2022 SRS imaging of lipid-associated mitochondrial dysfunction in human skeletal muscle

Lingyan Shi, Phyllis Chang, Audrey Zeng, Yajuan Li, Bryan Bergman

Proceedings Volume PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII; PC119650Q (2022) https://doi.org/10.1117/12.2614093
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We applied multiphoton fluorescence, SHG, and SRS to investigate In situ and chemical-specific identification of lipid and mitochondrial composition is crucial in analyzing the impacted subcellular components resulting in lipid accumulation, and differentiate mitochondrial associated bimolecular identification and related lipids’ spatial distribution in skeletal muscle cells between athletes and type 2 diabetes patients, to reveal differences in composition related to dysregulated metabolic pathways. Furthermore, quantitative SRS analysis revealed markers of irregular molecules for impacted mitochondrial function corresponding to decreased lipid metabolism seen in type 2 diabetes.

Conference Presentation

Citation Download Citation

Lingyan Shi, Phyllis Chang, Audrey Zeng, Yajuan Li, and Bryan Bergman "SRS imaging of lipid-associated mitochondrial dysfunction in human skeletal muscle", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC119650Q (7 March 2022); https://doi.org/10.1117/12.2614093

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