Optical gearbox enabled high-speed two-photon functional imaging

Meng Cui; Jianian Lin; Zongyue Cheng

doi:10.1117/12.2650264

17 March 2023 Optical gearbox enabled high-speed two-photon functional imaging

Meng Cui, Jianian Lin, Zongyue Cheng

Proceedings Volume PC12365, Neural Imaging and Sensing 2023; PC1236509 (2023) https://doi.org/10.1117/12.2650264
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Laser scanning two-photon fluorescence microscopy is widely used for in vivo neural recordings. Commercially available imaging systems can support an imaging rate of up to tens of Hz. With the emergence of faster function indicators, an imaging rate of hundreds of Hz is needed, which is beyond the capability of the widely used systems. To broadly enable such high-speed imaging capabilities in neuroscience research, we have developed an optical gearbox system that is compatible with the commonly available hardware systems. We employed the gearbox-based two-photon system for high-speed recording of neuronal and vascular dynamics in the animal brain.

Conference Presentation

Citation Download Citation

Meng Cui, Jianian Lin, and Zongyue Cheng "Optical gearbox enabled high-speed two-photon functional imaging", Proc. SPIE PC12365, Neural Imaging and Sensing 2023, PC1236509 (17 March 2023); https://doi.org/10.1117/12.2650264

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