Analysis of the different type collagen with pixel-resolution by Dual-LC PSHG microscopy (Conference Presentation)

Chi-Hsiang Lien; Anupama Nair; Shu‐Chun Chuang; Yi-Shan Lin; Chun-Yu Lin; Chung-Hwan Chen; Shean-Jen Chen

doi:10.1117/12.2650478

15 March 2023 Analysis of the different type collagen with pixel-resolution by Dual-LC PSHG microscopy (Conference Presentation)

Chi-Hsiang Lien, Anupama Nair, Shu‐Chun Chuang, Yi-Shan Lin, Chun-Yu Lin, Chung-Hwan Chen, Shean-Jen Chen

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Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840P (2023) https://doi.org/10.1117/12.2650478
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Collagen is ubiquitously found inside the human body and is the main structural protein in the Extracellular Matrix (ECM) of various tissues. In this work, we focus on the improvement instrumentation and methods used for obtaining collagen structural information in the SHG microscopy. We proposed dual liquid crystals based polarization-resolved SHG approach to address the deadlock, which breaks limitation of only working for modulating excitation angle of linear polarization but not directionality for all polarization state control. We assessed the SHG polarization excited responses of the isolated collagen type I and type II gels with quantitative analysis methods at different scale levels using our system and the results showed the potential of Dual-LC PSHG microscopy to differentiate the type of collagen based on the macroscopic SHG responses.

Conference Presentation

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Chi-Hsiang Lien, Anupama Nair, Shu‐Chun Chuang, Yi-Shan Lin, Chun-Yu Lin, Chung-Hwan Chen, and Shean-Jen Chen "Analysis of the different type collagen with pixel-resolution by Dual-LC PSHG microscopy (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840P (15 March 2023); https://doi.org/10.1117/12.2650478

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KEYWORDS

Collagen

Microscopy

Anisotropy

Polarization

Second-harmonic generation

Tissues

Liquid crystals

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