Optical tweezers assessment of local ECM mechanical heterogeneity during capillary morphogenesis

Michelle Lanterman; Irene Zhang; Sarah Eldeen; Ulysses Castillo; Andrew Putnam; Elliot Botvinick

doi:10.1117/12.2681249

5 October 2023 Optical tweezers assessment of local ECM mechanical heterogeneity during capillary morphogenesis

Michelle Lanterman, Irene Zhang, Sarah Eldeen, Ulysses Castillo, Andrew Putnam, Elliot Botvinick

Proceedings Volume PC12649, Optical Trapping and Optical Micromanipulation XX; PC126490T (2023) https://doi.org/10.1117/12.2681249
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

ECM stiffness is a potential instructive cue during capillary morphogenesis. Bulk measurements have often been used to investigate matrix stiffness as a cellular cue. However, local peri-cellular stiffness can be heterogeneous and vary greatly from measured bulk properties spatially and temporally. Here, we utilize bulk rheology and optical tweezers active microrheology (AMR) to investigate the dynamic mechanical crosstalk between cells and the surrounding matrix during capillary morphogenesis across length scales with various supporting stromal cells. We will present our current findings focusing on the discrepancies between bulk and microrheological distributions of measured stiffness across different stromal cell types.

Conference Presentation

Citation Download Citation

Michelle Lanterman, Irene Zhang, Sarah Eldeen, Ulysses Castillo, Andrew Putnam, and Elliot Botvinick "Optical tweezers assessment of local ECM mechanical heterogeneity during capillary morphogenesis", Proc. SPIE PC12649, Optical Trapping and Optical Micromanipulation XX, PC126490T (5 October 2023); https://doi.org/10.1117/12.2681249

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available