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The use of metallic nanoparticles in applications ranging from drug delivery to consumer electronics has exploded in the last two decades. Although this broad range of use cases has brought about technological revolutions in multiple fields, the effects of widespread production and subsequent human exposure to these nanoparticles have yet to be fully understood. New imaging techniques are a critical part of developing a more complete understanding of chronic exposure and biodistribution. Here we present a novel label free luminescence imaging technique to analyze the biodistribution, content, and biological context of metallic nanoparticles using multiphoton luminescence.
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Sean Burkitt, Anthony Mouchawar, Olga Eremina, Augusta Fernando, Cristina Zavaleta, "Label-free visualization and analysis of metallic nanoparticle biodistribution using multiphoton luminescence," Proc. SPIE PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, PC1282108 (13 March 2024); https://doi.org/10.1117/12.3007514