Label-free dynamic contrast imaging of cellular activity with Powell lens-based line-field OCT (PL-LF-dOCT)

Keyu Chen; Stephanie Swanson; Julian Parkin; Saduni Sumithrarachchige; Ernest Osei; Kostadinka Bizheva

doi:10.1117/12.3005707

13 March 2024 Label-free dynamic contrast imaging of cellular activity with Powell lens-based line-field OCT (PL-LF-dOCT)

Keyu Chen, Stephanie Swanson, Julian Parkin, Saduni Sumithrarachchige, Ernest Osei, Kostadinka Bizheva

Author Affiliations +

Proceedings Volume PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; PC1283027 (2024) https://doi.org/10.1117/12.3005707
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Dynamic OCT (dOCT) method uses time-depended OCT signal fluctuations arising from cellular motion in biological tissues to generate additional contrast in OCT images. So far dOCT studies have been conducted with Point-scanning and Full-field dOCT platforms, each of them offering advantages and limitations such as FOV, volumetric scan and phase stability. Here we present the first line-field dOCT platform (LF-dOCT), which allows low jitter B-scan dOCT signal collections. A Powell lens is utilized for uniform line-direction system sensitivity. Various biological tissues have been imaged with the LF-dOCT platform to validate its ability to detect cellular and intracellular dynamics.

Conference Presentation

Citation Download Citation

Keyu Chen, Stephanie Swanson, Julian Parkin, Saduni Sumithrarachchige, Ernest Osei, and Kostadinka Bizheva "Label-free dynamic contrast imaging of cellular activity with Powell lens-based line-field OCT (PL-LF-dOCT)", Proc. SPIE PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, PC1283027 (13 March 2024); https://doi.org/10.1117/12.3005707

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