Diagnostic evaluation and clinical concordance analysis of label-free photon absorption remote sensing virtual H&E staining in breast core biopsy samples

James Tweel; Benjamin R. Ecclestone; Hager Gaouda; Deepak Dinakaran; Michael P. Wallace; Gilbert Bigras; John R. Mackey; Parsin Haji Reza

doi:10.1117/12.3001158

13 March 2024 Diagnostic evaluation and clinical concordance analysis of label-free photon absorption remote sensing virtual H&E staining in breast core biopsy samples

James Tweel, Benjamin R. Ecclestone, Hager Gaouda, Deepak Dinakaran, Michael P. Wallace, Gilbert Bigras, John R. Mackey, Parsin Haji Reza

Author Affiliations +

Proceedings Volume PC12831, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII; PC1283106 (2024) https://doi.org/10.1117/12.3001158
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We compared the diagnostic accuracy of label-free Photon Absorption Remote Sensing (PARS) virtual hematoxylin and eosin (H&E) staining to conventional histology in breast cancer core needle biopsy (CNB) samples. Matching PARS virtual H&E and real H&E images were acquired for seven breast CNB samples representing a spectrum of histologies. Seven board certified pathologists, blinded to the image origin, provided diagnostic evaluations of each sample. A kappa concordance analysis was performed and revealed 'substantial' agreement (kappa > 0.6) between the PARS and H&E pairs in primary diagnosis. Pathologists were unable to distinguish the true origin of the PARS virtual H&E images.

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James Tweel, Benjamin R. Ecclestone, Hager Gaouda, Deepak Dinakaran, Michael P. Wallace, Gilbert Bigras, John R. Mackey, and Parsin Haji Reza "Diagnostic evaluation and clinical concordance analysis of label-free photon absorption remote sensing virtual H&E staining in breast core biopsy samples", Proc. SPIE PC12831, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII, PC1283106 (13 March 2024); https://doi.org/10.1117/12.3001158

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