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Optical Photothermal Infrared (O-PTIR) is a rapidly emerging technique for super-resolution infrared spectroscopy that uses a short wavelength visible probe beam to detect infrared absorption by a sample with 10-30X better spatial resolution than conventional infrared spectroscopy. O-PTIR has been used in biomedical applications including cancer, neurodegenerative disease, cellular metabolism research, and other areas. O-PTIR has recently been combined fluorescence imaging to provide targeted IR spectroscopic analysis of fluorescently labeled regions of cells and tissue and also to rapidly detect IR absorption over a wide sample area. This presentation will overview O-PTIR technology and survey a variety of applications.
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