Fluorescence-encoded low-frequency vibrational spectroscopy in the visible region

Tetsu Tamura; Phillip Charles McCann; Kotaro Hiramatsu; Keisuke Goda

doi:10.1117/12.2692410

13 March 2024 Fluorescence-encoded low-frequency vibrational spectroscopy in the visible region

Tetsu Tamura, Phillip Charles McCann, Kotaro Hiramatsu, Keisuke Goda

Proceedings Volume PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry; PC128390S (2024) https://doi.org/10.1117/12.2692410
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Here, we present vFLETCHERS (visible fluorescence-encoded time-domain coherent Raman spectroscopy), which operates in the visible excitation region and overcomes the previous limitation of detectable fluorophores. vFLETCHERS employs a non-collinear optical parametric amplifier as a femtosecond excitation source. As a proof-of-concept demonstration, we acquired low-frequency Raman spectra (<1000 cm^-1) of solutions containing commercial fluorophores with the absorption peaks in the 600-700 nm region. These results highlight the potential of vFLETCHERS as a versatile multiplexed imaging technique, opening up new opportunities for research in biology.

Conference Presentation

Citation Download Citation

Tetsu Tamura, Phillip Charles McCann, Kotaro Hiramatsu, and Keisuke Goda "Fluorescence-encoded low-frequency vibrational spectroscopy in the visible region", Proc. SPIE PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry, PC128390S (13 March 2024); https://doi.org/10.1117/12.2692410

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