Enabling virus detection via Raman spectroscopy with magnetic beads as a tool for sample preparation

Susanne Pahlow; Marie Richard-Lacroix; Franziska Hornung; Lea Herrmann; Ziliang Zhao; Stefanie Reuter; Nilay Köse-Vogel; Thomas Mayerhöfer; Julian Hniopek; Oleg Ryabchykov; Thomas Bocklitz; Karina Weber; Ralf Mrowka; Ralf Ehricht; Bettina Löffler; Christian Eggeling; Stefanie Deinhardt-Emmer; Jürgen Popp

doi:10.1117/12.3012033

13 March 2024 Enabling virus detection via Raman spectroscopy with magnetic beads as a tool for sample preparation

Susanne Pahlow, Marie Richard-Lacroix, Franziska Hornung, Lea Herrmann, Ziliang Zhao, Stefanie Reuter, Nilay Köse-Vogel, Thomas Mayerhöfer, Julian Hniopek, Oleg Ryabchykov, Thomas Bocklitz, Karina Weber, Ralf Mrowka, Ralf Ehricht, Bettina Löffler, Christian Eggeling, Stefanie Deinhardt-Emmer, Jürgen Popp

Author Affiliations +

Proceedings Volume PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry; PC128390T (2024) https://doi.org/10.1117/12.3012033
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We developed a simple and convenient magnetic bead-based sample preparation scheme for enabling a Raman spectroscopic differentiation of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) positive and negative samples. By utilizing the angiotensin-converting enzyme 2 (ACE2) receptor protein as selective recognition element, we avoid having to identify the virus species based on its specific Raman signature. Instead we only need to verify the presence of the virus, which is significantly less difficult. For quantitative evaluation of the spectra, we calculated the Pearson coefficient and the Normalized Cross Correlation coefficient.

Conference Presentation

Citation Download Citation

Susanne Pahlow, Marie Richard-Lacroix, Franziska Hornung, Lea Herrmann, Ziliang Zhao, Stefanie Reuter, Nilay Köse-Vogel, Thomas Mayerhöfer, Julian Hniopek, Oleg Ryabchykov, Thomas Bocklitz, Karina Weber, Ralf Mrowka, Ralf Ehricht, Bettina Löffler, Christian Eggeling, Stefanie Deinhardt-Emmer, and Jürgen Popp "Enabling virus detection via Raman spectroscopy with magnetic beads as a tool for sample preparation", Proc. SPIE PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry, PC128390T (13 March 2024); https://doi.org/10.1117/12.3012033

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available