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13 March 2024 Label-free optical imaging probing of metabolic states during human pluripotent stem cell differentiation to definitive endoderm
Maria Savvidou, Demetrios M. Stoukides, Yang Zhang, Emmanuel S. Tzanakakis, Irene Georgakoudi
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Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285402 (2024) https://doi.org/10.1117/12.3003494
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
The efficient transition of hPSCs to definitive endoderm (DE) progeny is an essential step toward disease modeling and the manufacturing of a wide range of cellular therapeutics in medical relevant quantities. Two-photon excited fluorescence (TPEF) imaging, as a non-invasive, non-destructive, label-free modality for metabolic studies, reveals the distinct metabolic switches during DE differentiation in a real-time monitoring mode. Since metabolic pathways orchestrate important regulatory mechanisms that influence and determine cell fate decisions, TPEF imaging serves as an important enabling technology in hPSC-based tissue engineering applications affording non-invasive determination of metabolic biomarkers and informing optimizations of hPSCs differentiation processes.
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Maria Savvidou, Demetrios M. Stoukides, Yang Zhang, Emmanuel S. Tzanakakis, and Irene Georgakoudi "Label-free optical imaging probing of metabolic states during human pluripotent stem cell differentiation to definitive endoderm", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285402 (13 March 2024); https://doi.org/10.1117/12.3003494
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KEYWORDS
Stem cells
Optical imaging
Fluorescence
Image display
Liver
Lung
Mitochondria
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