Presentation
13 March 2024 Structural, biochemical and biomechanical changes in collagenous tissues upon digestion and cross-linking monitored by combined optical and force microscopy imaging techniques
Tanveer A. Shaik, João L. Lagarto, Enrico Baria, Xinyue Wang, Christiane Höppener, Melis Goktas, Kerstin Blank, Volker Deckert, Francesco S. Pavone, Juergen Popp, Christoph Krafft, Riccardo Cicchi
Author Affiliations +
Abstract
We demonstrate the capability of linear and non-linear optical microspectroscopy techniques such as fluorescence lifetime imaging (FLIM), Raman spectroscopy (RS), second-harmonic generation (SHG), and two-photon fluorescence (TPF), to monitor structural, biochemical and biomechanical alterations in collagenous tissues upon both collagen digestion and cross-linking using genipin as cross-linker. Thanks to a unique combination of optical and force microscopy techniques in a correlative manner, we obtained detailed information about the biochemical, structural, and biomechanical properties of collagenous tissues upon both digestion and cross-linking treatment.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tanveer A. Shaik, João L. Lagarto, Enrico Baria, Xinyue Wang, Christiane Höppener, Melis Goktas, Kerstin Blank, Volker Deckert, Francesco S. Pavone, Juergen Popp, Christoph Krafft, and Riccardo Cicchi "Structural, biochemical and biomechanical changes in collagenous tissues upon digestion and cross-linking monitored by combined optical and force microscopy imaging techniques", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540Z (13 March 2024); https://doi.org/10.1117/12.3002353
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KEYWORDS
Biomechanics

Collagen

Optical microscopy

Optical sensing

Raman spectroscopy

Second harmonic generation

Tissue optics

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