Structural, biochemical and biomechanical changes in collagenous tissues upon digestion and cross-linking monitored by combined optical and force microscopy imaging techniques

Tanveer A. Shaik; João L. Lagarto; Enrico Baria; Xinyue Wang; Christiane Höppener; Melis Goktas; Kerstin Blank; Volker Deckert; Francesco S. Pavone; Juergen Popp; Christoph Krafft; Riccardo Cicchi

doi:10.1117/12.3002353

13 March 2024 Structural, biochemical and biomechanical changes in collagenous tissues upon digestion and cross-linking monitored by combined optical and force microscopy imaging techniques

Tanveer A. Shaik, João L. Lagarto, Enrico Baria, Xinyue Wang, Christiane Höppener, Melis Goktas, Kerstin Blank, Volker Deckert, Francesco S. Pavone, Juergen Popp, Christoph Krafft, Riccardo Cicchi

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540Z (2024) https://doi.org/10.1117/12.3002353
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We demonstrate the capability of linear and non-linear optical microspectroscopy techniques such as fluorescence lifetime imaging (FLIM), Raman spectroscopy (RS), second-harmonic generation (SHG), and two-photon fluorescence (TPF), to monitor structural, biochemical and biomechanical alterations in collagenous tissues upon both collagen digestion and cross-linking using genipin as cross-linker. Thanks to a unique combination of optical and force microscopy techniques in a correlative manner, we obtained detailed information about the biochemical, structural, and biomechanical properties of collagenous tissues upon both digestion and cross-linking treatment.

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Tanveer A. Shaik, João L. Lagarto, Enrico Baria, Xinyue Wang, Christiane Höppener, Melis Goktas, Kerstin Blank, Volker Deckert, Francesco S. Pavone, Juergen Popp, Christoph Krafft, and Riccardo Cicchi "Structural, biochemical and biomechanical changes in collagenous tissues upon digestion and cross-linking monitored by combined optical and force microscopy imaging techniques", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540Z (13 March 2024); https://doi.org/10.1117/12.3002353

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