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Fluorescence microscopy relies on efficient emission of molecules excited by an incident laser light. However, their emission is often intrinsically limited by a low fluorescence quantum yield which results in low contrast images. In our recent efforts, we explored the fluorescence life-time imaging contrast for those low efficient fluorophores to find out that those molecules exhibit a rather intense emission over a relatively short time. This short fluorescence time was found to be dependent of the local structure and local environment providing a novel biomarker for biological imaging.
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Vladislav V. Yakovlev, Vsevolod Cheburkanov, Jason W. Pipal, "Low quantum yield fluorophores: an obstacle or an opportunity?," Proc. SPIE PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, PC1286208 (13 March 2024); https://doi.org/10.1117/12.3006260