3D-printed organoid phantoms: a tool for developing quantitative phase imaging methods for highly-scattering samples

Arkadiusz T. Kus; Michal Ziemczonok; Cécile Fiche; Sylvia Desissaire; Clement Acquitter; Lionel Hervé; Marie Fackeure; Jeremy Neri; Wojciech Krauze; Kiran Padmanabhan; Chiara Paviolo

doi:10.1117/12.3017673

18 June 2024 3D-printed organoid phantoms: a tool for developing quantitative phase imaging methods for highly-scattering samples

Arkadiusz T. Kus, Michal Ziemczonok, Cécile Fiche, Sylvia Desissaire, Clement Acquitter, Lionel Hervé, Marie Fackeure, Jeremy Neri, Wojciech Krauze, Kiran Padmanabhan, Chiara Paviolo

Author Affiliations +

Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC1299607 (2024) https://doi.org/10.1117/12.3017673
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

One of the directions of development in quantitative phase imaging is to provide the capability to reconstruct the phase or preferably refractive index (RI) distribution within thick, highly scattering samples. This direction coincides with current trends in biology, where three-dimensional (3D) organoids are currently replacing standard 2D cultures as more physiological models for tissue growth and organ formation in a dish. The biological complexity of these 3D structures makes the imaging and RI reconstruction particularly challenging, and thus calibration as well as validation structures are important and sought-after tools in instrumentation development. For this reason, in this work, we present the full preparation and measurement procedure for organoid phantoms printed with two-photon polymerization along with the method to obtain the ground truth of the object structure independently of RI reconstruction errors and artifacts.

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Arkadiusz T. Kus, Michal Ziemczonok, Cécile Fiche, Sylvia Desissaire, Clement Acquitter, Lionel Hervé, Marie Fackeure, Jeremy Neri, Wojciech Krauze, Kiran Padmanabhan, and Chiara Paviolo "3D-printed organoid phantoms: a tool for developing quantitative phase imaging methods for highly-scattering samples", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC1299607 (18 June 2024); https://doi.org/10.1117/12.3017673

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