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24 July 2024 Fast quantitative phase imaging with an LED array microscope using a perturbative approach
Jonathan Dong, Kuan-Chen Shen, Michael Unser, Laura Waller, Ruiming Cao
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Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC129960D (2024) https://doi.org/10.1117/12.3022419
Event: SPIE Photonics Europe, 2024, Strasbourg, France
Abstract
Quantitative Phase Imaging is the method of choice to observe unlabelled biological cells, enabling the exploration of their intricate structures and dynamics. Differential Phase Contrast (DPC) is a fast and flexible approach but is limited to brightfield measurements, which limits the achievable resolution. In this study, we revisit DPC as a perturbative approach for high-resolution phase imaging with a minimal number of images. Our methodology combines both a perturbative phase retrieval algorithm and ring-shaped darkfield illumination patterns. The proposed approach leverages the advantages of DPC, such as its minimal image requirement, while addressing its inability to extend to darkfield imaging. By reducing the number of acquired images, our technique offers a rapid solution for examining dynamic systems.
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Jonathan Dong, Kuan-Chen Shen, Michael Unser, Laura Waller, and Ruiming Cao "Fast quantitative phase imaging with an LED array microscope using a perturbative approach", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC129960D (24 July 2024); https://doi.org/10.1117/12.3022419
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KEYWORDS
Phase imaging
Imaging arrays
Light emitting diodes
Microscopes
Biological imaging
Image processing
Phase contrast
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