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4 September 2018 Optical characterization of two cyanobacteria genera, Aphanizomenon and Microcystis, with hyperspectral microscopy
Emily C. Paine, E. Terrence Slonecker, Nancy S. Simon, Barry H. Rosen, Ronald G. Resmini, David W. Allen
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Journal of Applied Remote Sensing, Vol. 12, Issue 3, 036013 (September 2018). https://doi.org/10.1117/1.JRS.12.036013
Abstract
Cyanobacterial blooms are a nuisance and a potential hazard in freshwater systems worldwide. Remote sensing has been used to detect cyanobacterial blooms, but few studies have distinguished among genera of cyanobacteria. Because some genera are more likely to be toxic than others, this is a useful distinction. Hyperspectral imaging reflectance microscopy was used to examine cyanobacteria from Upper Klamath Lake, Oregon, at high spatial and spectral resolution to determine if two species found commonly in the lake, Aphanizomenon flos-aquae and Microcystis aeruginosa, can be separated spectrally. Of the analytical methods applied, a spectral shape algorithm applied to the derivative was found to be most successful in classifying these species in microscope scenes. Further work is required to determine if the spectral characterization of cyanobacterial genera can be scaled up to remote sensing applications.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Emily C. Paine, E. Terrence Slonecker, Nancy S. Simon, Barry H. Rosen, Ronald G. Resmini, and David W. Allen "Optical characterization of two cyanobacteria genera, Aphanizomenon and Microcystis, with hyperspectral microscopy," Journal of Applied Remote Sensing 12(3), 036013 (4 September 2018). https://doi.org/10.1117/1.JRS.12.036013
Received: 17 August 2017; Accepted: 14 August 2018; Published: 4 September 2018
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Cited by 10 scholarly publications.
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KEYWORDS
Microscopes
Algorithm development
Hyperspectral imaging
Microscopy
Remote sensing
Reflectivity
Absorption
CHORUS Article. This article was made freely available starting 04 September 2019
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