CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 13 > Issue 3 > Article
Open Access
1 May 2008 Fluorescent protein tools for studying protein dynamics in living cells: a review
Richard N. Day, Fred Schaufele
Author Affiliations +
Journal of Biomedical Optics, Vol. 13, Issue 3, 031202 (May 2008). https://doi.org/10.1117/1.2939093
Abstract
We have witnessed remarkable advances over the past decade in the application of optical techniques to visualize the genetically encoded fluorescent proteins (FPs) in living systems. The imaging of the FPs inside living cells has become an essential tool for studies of cell biology and physiology. FPs are now available that span the visible spectrum from deep blue to deep red, providing a wide choice of genetically encoded fluorescent markers. Furthermore, some FPs have been identified that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells. These additions to the FP toolbox are now being used for some very innovative live-cell imaging applications. Here, we will highlight the characteristics and uses of a few of these exceptional probes. Many different optical methods can be combined with the FPs from marine organisms to provide quantitative measurements in living systems.
©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation Download Citation
Richard N. Day and Fred Schaufele "Fluorescent protein tools for studying protein dynamics in living cells: a review," Journal of Biomedical Optics 13(3), 031202 (1 May 2008). https://doi.org/10.1117/1.2939093
Published: 1 May 2008
JOURNAL ARTICLE
 6 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 73 scholarly publications and 9 patents.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Proteins
Fluorescent proteins
Fluorescence resonance energy transfer
Green fluorescent protein
Luminescence
Cell biology
Ocean optics
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top