Measuring reactive hyperemia in the lower limb using near-infrared spectroscopy

Thomas B. B. Willingham; William M. Southern; Kevin K. McCully

doi:10.1117/1.JBO.21.9.091302

5 April 2016 Measuring reactive hyperemia in the lower limb using near-infrared spectroscopy

Thomas B. B. Willingham, William M. Southern, Kevin K. McCully

Author Affiliations +

Journal of Biomedical Optics, Vol. 21, Issue 9, 091302 (April 2016). https://doi.org/10.1117/1.JBO.21.9.091302

Abstract

Near-infrared spectroscopy (NIRS) has been used to measure reactive hyperemia following a vascular occlusion. However, the procedures and methods of analysis used have varied. The purpose of the present study is to identify reproducible methods for measuring reactive hyperemia using HbO₂ NIRS signals in the calf and foot. Healthy participants (10 male, 10 female) aged 19 to 28 years performed one of two tests: reproducibility trials or elevation protocol (30 and 60 cm limb elevation above the heart). The time to 50% reperfusion (T_1/2) and the second (R_2q) quartile rates of reperfusion were found to be the most reproducible parameters (coefficient of variation=7.12 to 14.1%). The time to 95% reperfusion (T₉₅) was 12.7% more reproducible on average than the previously reported parameter of time to peak hyperemia. Measures of reperfusion time and rate slowed with increasing limb elevation. Correlations were identified between the calf and foot in the measurements of R_2q (R²=0.713, p=0.021), T_1/2 (R²=0.673, p=0.033), and T₉₅ (R²=0.792, p=0.006). Half and 95% recovery times and second and third quartile rates expressed good reproducibility and sensitivity to change with reduced perfusion pressure. NIRS measures of reactive hyperemia have the potential to evaluate microvascular perfusion in clinical populations.

Citation Download Citation

Thomas B. B. Willingham, William M. Southern, and Kevin K. McCully "Measuring reactive hyperemia in the lower limb using near-infrared spectroscopy," Journal of Biomedical Optics 21(9), 091302 (5 April 2016). https://doi.org/10.1117/1.JBO.21.9.091302

Published: 5 April 2016

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY