Microfluidic velocity measurement using a scanning laser Doppler microscope

Han-Sheng Chuang; Yu-Lung Lo

doi:10.1117/1.2673887

1 February 2007 Microfluidic velocity measurement using a scanning laser Doppler microscope

Han-Sheng Chuang, Yu-Lung Lo

Author Affiliations +

Optical Engineering, Vol. 46, Issue 2, 024301 (February 2007). https://doi.org/10.1117/1.2673887

Abstract

This study develops a scanning laser Doppler microscope (LDM) to measure the velocity flow profile within a capillary. The flow velocity is established by detecting the periodic fluctuation in the scattered light as the microspheres pass through a measurement volume with a diameter of 15 μm. Using a programmable positioning stage, the position of the capillary relative to the focal point is shifted incrementally in the x- and y-axis directions, respectively, such that the velocity flow profiles in the X-X′ and Y-Y′ directions can be obtained. During scanning, the required stage displacements in the x- and y-axis directions are calculated using an analytical model that compensates for deviations in the focal point position caused by the mismatched refractive index effect. The velocity–flow rate relationship within a capillary with an internal diameter of 75 μm is characterized and is found to be in good agreement with the theoretical prediction [correlation coefficient (R2): 0.998]. Additionally, the measured velocity flow profiles in the X-X′ and Y-Y′ directions are in good agreement with the analytical results obtained from the Navier-Stokes equation. The scanning LDM provides a powerful technique for characterizing microfluidic flow fields in the cross section of micro-electro-mechanical systems–based biochips and similar advanced applications.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Han-Sheng Chuang and Yu-Lung Lo "Microfluidic velocity measurement using a scanning laser Doppler microscope," Optical Engineering 46(2), 024301 (1 February 2007). https://doi.org/10.1117/1.2673887

Published: 1 February 2007

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE