The goal of this project was to construct a cart and a mounting system that would allow a hyperspectral laser-induced fluorescence imaging system (HLIFIS) to be used to detect fecal material in produce fields. Fecal contaminated produce is a recognized food safety risk. Previous research demonstrated the HLIFIS could detect fecal contamination in a laboratory setting. A cart was designed and built, and then tested to demonstrate that the cart was capable of moving at constant speeds or at precise intervals. A mounting system was designed and built to facilitate the critical alignment of the camera’s imaging and the laser’s illumination fields, and to allow the HLIFIS to be used in both field and laboratory settings without changing alignments. A hardened mount for the Powell lens that is used to produce the appropriate illumination profile was also designed, built, and tested.
Unmanned ground vehicles have been utilized in the last few decades in an effort to increase the efficiency of agriculture, in particular, by reducing labor needs. Unmanned vehicles have been used for a variety of purposes including: soil sampling, irrigation management, precision spraying, mechanical weeding, and crop harvesting. In this paper, unmanned ground vehicles, implemented by researchers or commercial operations, are characterized through a comparison to other vehicles used in agriculture, namely airplanes and UAVs. An overview of different trade-offs of configurations, control schemes, and data collection technologies is provided. Emphasis is given to the use of unmanned ground vehicles in food crops, and includes a discussion of environmental impacts and economics. Factors considered regarding the future trends and potential issues of unmanned ground vehicles include development, management and performance. Also included is a strategy to demonstrate to farmers the safety and profitability of implementing the technology.
For sanitation inspection in food processing environment, fluorescence imaging can be a very useful method because many organic materials reveal unique fluorescence emissions when excited by UV or violet radiation. Although some fluorescence-based automated inspection instrumentation has been developed for food products, there remains a need for devices that can assist on-site inspectors performing visual sanitation inspection of the surfaces of food processing/handling equipment. This paper reports the development of an inexpensive handheld imaging device designed to visualize fluorescence emissions and intended to help detect the presence of fecal contaminants, organic residues, and bacterial biofilms at multispectral fluorescence emission bands. The device consists of a miniature camera, multispectral (interference) filters, and high power LED illumination. With WiFi communication, live inspection images from the device can be displayed on smartphone or tablet devices. This imaging device could be a useful tool for assessing the effectiveness of sanitation procedures and for helping processors to minimize food safety risks or determine potential problem areas. This paper presents the design and development including evaluation and optimization of the hardware components of the imaging devices.
This research developed and evaluated the multispectral algorithms derived from hyperspectral line-scan fluorescence
imaging under violet LED excitation for detection of fecal contamination on Golden Delicious apples. The algorithms
utilized the fluorescence intensities at four wavebands, 680 nm, 684 nm, 720 nm, and 780 nm, for computation of simple
functions for effective detection of contamination spots created on the apple surfaces using four concentrations of
aqueous fecal dilutions. The algorithms detected more than 99% of the fecal spots. The effective detection of feces
showed that a simple multispectral fluorescence imaging algorithm based on violet LED excitation may be appropriate
to detect fecal contamination on fast-speed apple processing lines.
This paper reported the development of hyperspectral fluorescence imaging system using ultraviolet-A excitation (320-400 nm) for detection of bovine fecal contaminants on the abaxial and adaxial surfaces of romaine lettuce and baby
spinach leaves. Six spots of fecal contamination were applied to each of 40 lettuce and 40 spinach leaves. In this study,
the wavebands at 666 nm and 680 nm were selected by the correlation analysis. The two-band ratio, 666 nm / 680 nm, of
fluorescence intensity was used to differentiate the contaminated spots from uncontaminated leaf area. The proposed
method could accurately detect all of the contaminated spots.
We used a portable hyperspectral fluorescence imaging system to evaluate biofilm formations on four types of food
processing surface materials including stainless steel, polypropylene used for cutting boards, and household counter top
materials such as formica and granite. The objective of this investigation was to determine a minimal number of spectral
bands suitable to differentiate microbial biofilm formation from the four background materials typically used during
food processing. Ultimately, the resultant spectral information will be used in development of handheld portable
imaging devices that can be used as visual aid tools for sanitation and safety inspection (microbial contamination) of the
food processing surfaces. Pathogenic E. coli O157:H7 and Salmonella cells were grown in low strength M9 minimal
medium on various surfaces at 22 ± 2 °C for 2 days for biofilm formation. Biofilm autofluorescence under UV
excitation (320 to 400 nm) obtained by hyperspectral fluorescence imaging system showed broad emissions in the blue-green
regions of the spectrum with emission maxima at approximately 480 nm for both E. coli O157:H7 and Salmonella
biofilms. Fluorescence images at 480 nm revealed that for background materials with near-uniform fluorescence
responses such as stainless steel and formica cutting board, regardless of the background intensity, biofilm formation can
be distinguished. This suggested that a broad spectral band in the blue-green regions can be used for handheld imaging
devices for sanitation inspection of stainless, cutting board, and formica surfaces. The non-uniform fluorescence
responses of granite make distinctions between biofilm and background difficult. To further investigate potential
detection of the biofilm formations on granite surfaces with multispectral approaches, principal component analysis
(PCA) was performed using the hyperspectral fluorescence image data. The resultant PCA score images revealed
distinct contrast between biofilms and granite surfaces. This investigation demonstrated that biofilm formations on food
processing surfaces, even for background materials with heterogeneous fluorescence responses, can be
detected. Furthermore, a multispectral approach in developing handheld inspection devices may be needed to inspect
surface materials that exhibit non-uniform fluorescence.
Automated imaging systems offer the potential to inspect the quality and safety of fruits and vegetables consumed by the public. Current automated inspection systems allow fruit such as apples to be sorted for quality issues including color and size by looking at a portion of the surface of each fruit. However, to inspect for defects and contamination, the whole surface of each fruit must be imaged. The goal of this project was to develop an effective and economical method for whole surface imaging of apples using mirrors and a single camera. Challenges include mapping the concave stem and calyx regions. To allow the entire surface of an apple to be imaged, apples were suspended or rolled above the mirrors using two parallel music wires. A camera above the apples captured 90 images per sec (640 by 480 pixels). Single or multiple flat or concave mirrors were mounted around the apple in various configurations to maximize surface imaging. Data suggest that the use of two flat mirrors provides inadequate coverage of a fruit but using two parabolic concave mirrors allows the entire surface to be mapped. Parabolic concave mirrors magnify images, which results in greater pixel resolution and reduced distortion. This result suggests that a single camera with two parabolic concave mirrors can be a cost-effective method for whole surface imaging.
We have developed nondestructive opto-electronic imaging techniques for rapid assessment of safety and
wholesomeness of foods. A recently developed fast hyperspectral line-scan imaging system integrated with a
commercial apple-sorting machine was evaluated for rapid detection of animal feces matter on apples. Apples
obtained from a local orchard were artificially contaminated with cow feces. For the online trial, hyperspectral
images with 60 spectral channels, reflectance in the visible to near infrared regions and fluorescence emissions with
UV-A excitation, were acquired from apples moving at a processing sorting-line speed of three apples per second.
Reflectance and fluorescence imaging required a passive light source, and each method used independent continuous
wave (CW) light sources. In this paper, integration of the hyperspectral imaging system with the commercial applesorting
machine and preliminary results for detection of fecal contamination on apples, mainly based on the
fluorescence method, are presented.
Development of machine vision systems to examine fruit for quality and contamination problems has been stalled due
to lack of an inexpensive, fast, method for appropriately orienting fruit for imaging. We recently discovered that apples
could be oriented based-on inertial properties. Apples were rolled down a ramp consisting of two parallel rails. When
sufficient angular velocity was achieved, the apples moved to a configuration where the stem/calyx axis was
perpendicular to the direction of travel. This discovery provides a potential basis for development of a commercially-viable
orientation system. However, many question remain concerning the underlying dynamic principles that govern
this phenomenon. An imaging system and software were constructed to allow detailed observation of the orientation
process. Sequential 640×480 monochrome images are acquired at 60 fps and 1/500 sec exposure. The software finds the
center of the apple in each image as well as the vertical movement of the track at a selected coordinate. Early tests
revealed that the compliance of the track played a significant role in the orientation process. These data will be used to
compare results from empirical tests with predictions of dynamic models.
Our laboratory has been utilizing fluorescence techniques as a potential means for detection of quality and wholesomeness of food products. A system with a short pulse light source such as a laser coupled with a gated detector can be used to harvest fluorescence in ambient light conditions from biological samples with relatively low fluorescence yields. We present a versatile multispectral laser-induced fluorescence (LIF) imaging system capable of ns-scale time resolved fluorescence. The system is equipped with a tunable pulse laser system that emits in the visible range from 410 nm to 690 nm. Ns-scale, time-dependent multispectral fluorescence emissions of apples contaminated with a range of diluted cow feces were acquired. Four spectral bands, F670, F680, F685 and F730, centered near the emission peak wavelengths of the major constituents responsible for the red fluorescence emissions from apples artificially contaminated with cow feces were examined to determine a suitable single red fluorescence band and optimal ns-gate window for detection of fecal contamination on apples. The results based on the ns decay curves showed that 670 nm with 10 nm full width at half maximum (FWHM) at a gate-delay of 4 ns from the laser excitation peak provided the greatest differences in time-dependent fluorescence responses between feces contaminated spots and apples surfaces.
The use of spectral sensing has gained acceptance as a rapid means for nondestructive inspection of postharvest food
produce. Current technologies generally use color or a single wavelength camera technology. The applicability and
sensitivity of these techniques can be expanded through the use of multiple wavelengths. Reflectance in the
Vis/NIR is the prevalent spectral technique. Fluorescence, compared to reflectance, is regarded as a more sensitive
technique due to its dynamic responses to subtle changes in biological entities. Our laboratory has been exploring
fluorescence as a potential means for detection of quality and wholesomeness of food products. Applications of
fluorescence sensing require an understanding of the spectral characteristics emanating from constituents and
potential contaminants. A number of factors affecting fluorescence emission characteristics are discussed. Because
of relatively low fluorescence quantum yield from biological samples, a system with a powerful pulse light source
such as a laser coupled with a gated detection device is used to harvest fluorescence, in the presence of ambient
light. Several fluorescence sensor platforms developed in our laboratory, including hyperspectral imaging, and
laser-induced fluorescence (LIF) and steady-state fluorescence imaging systems with multispectral capabilities are
presented. We demonstrate the potential uses of recently developed fluorescence imaging platforms in food safety
inspection of apples contaminated with animal feces.
Fluorescence can be a sensitive method for detecting food contaminants. Of particular interest is detection of fecal
contamination as feces is the source of many pathogenic organisms. Feces generally contain chlorophyll a and related
compounds due to ingestion of plant materials, and these compounds can readily be detected using fluorescence
techniques. Described is a fluorescence-imaging system consisting primarily of a UV light source, an intensified camera
with a six-position filter wheel, and software for controlling the system and automatically analyzing the resulting
images. To validate the system, orchard apples artificially contaminated with dairy feces were used in a "hands-on"
public demonstration. The contamination sites were easily identified using automated edge detection and threshold
detection algorithms. In addition, by applying feces to apples and then washing sets of apples at hourly intervals, it was
determined that five h was the minimum contact time that allowed identification of the contamination site after the
apples were washed. There are many potential uses for this system, including studying the efficacy of apple washing
systems.
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