Ms. Alexandra A. Pashkevich Profile

Alexandra A. Pashkevich

at National Research Nuclear Univ MEPhI

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Proceedings Article | 12 September 2021 Presentation + Paper

Photon counting mode for advanced real-time detection of bioaerosols

Artem Akmalov, Alexander Chistyakov, Gennadii Kotkovskii, Yury Kuzishchin, Igor Martynov, Evgenii Osipov, Alexandra Pashkevich, Artem Tkachuk, Bakhtiyar Verdiev, Artem Alatyrev

Proceedings Volume 11869, 1186906 (2021) https://doi.org/10.1117/12.2600476

KEYWORDS: Atmospheric particles, Photodetectors, Aerosols, Luminescence, Light emitting diodes, Curium, Photon counting, Particles, Analog electronics, Signal detection

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Currently, one of the most important application of flow cytometry is the real-time analysis of aerosols, in particular, to ensure biosafety. In most cases, such analysis is aimed at detecting fluorescent signals from aerosol particles corresponding to the light emission of tryptophan and nicotinamide adenine dinucleotide (NADH). Further development of the method is largely related to the improvement of the light detecting systems for recording and processing of fluorescence and scattered light signals. In this work, a comparative analysis of flow cytometers for bioaerosols detection based on photo-multiplier tubes (PMT) and avalanche photodiodes (APD) operating in analog and photon-counting modes was carried out. The limit of detection (LOD) of bioaerosols, response time and ability to detect particles with low scattering and fluorescence cross section were calculated and examined. The calculations were carried out for the well-known optical scheme of fluorescence detection based on discrete photodetectors and dichroic mirrors combined with an air flow chamber equipped with elliptical and spherical mirrors. An ultraviolet light emission diode (LED) was used as a model source of exciting radiation. To estimate the optical properties of aerosol particles, experimental results obtained for a model bovine serum albumin bioaerosol and published data on various other bioaerosols were used. The calculation of the total number of fluorescent photons, emitted by particles of various sizes while passing the flow chamber was carried out. The obtained data were compared with parameters of photodetectors operating in analog and photon-counting modes. The critical particle size was determined for the effective registration in a photon-counting mode. Considering the size distribution of aerosol particles, it was concluded that application of the photon-counting mode will reduce the LOD of bioaerosols by more than an order of magnitude.

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