Prof. Allison M. Dennis Profile

Prof. Allison M. Dennis

Associate Professor at Northeastern Univ

SPIE Involvement:

Conference Program Committee | Editorial Board Member: Biophotonics Discovery | Author

Publications (20)

Proceedings Article | 13 March 2024 Presentation + Paper

Quantitative multiplexed SWIR imaging to study nanomedicine surface coatings in vivo

Xingjian Zhong, Amish Patel, Yidan Sun, Mallory Moffett, Allison Dennis

Proceedings Volume 12859, 1285902 (2024) https://doi.org/10.1117/12.3003317

KEYWORDS: Colloidal quantum dots, Nanoparticles, Biological imaging, Short wave infrared radiation, Multiplexing, Lymphatic system, Fluorescence imaging, In vivo imaging

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Proceedings Article | 13 March 2024 Presentation

Multispectral SWIR imaging with quantum dots highlights the mouse anatomy

Allison Dennis

Proceedings Volume PC12862, PC128620O (2024) https://doi.org/10.1117/12.3008374

KEYWORDS: Multispectral imaging, Quantum imaging, Biological imaging, Short wave infrared radiation, Semiconductor quantum dots, Anatomy, Visualization, Lymphatic system, Color, Tissues

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Proceedings Article | 13 March 2024 Presentation

ZnSe/InP/ZnS core-shell-shell quantum dots enable paired agent imaging in NIR-I window

Amish Patel, Allison Dennis

Proceedings Volume PC12859, PC1285905 (2024) https://doi.org/10.1117/12.3001869

KEYWORDS: Windows, Quantum dots, Quantum imaging, Zinc selenide, In vitro testing, Contrast agents, Zinc sulfide, Water, Tumors, Solubility

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SPIE Journal Paper | 5 April 2023 Open Access

Minimizing near-infrared autofluorescence in preclinical imaging with diet and wavelength selection

Yidan Sun, Xingjian Zhong, Allison M. Dennis

JBO, Vol. 28, Issue 09, 094805, (April 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.9.094805

KEYWORDS: Autofluorescence, Emission wavelengths, Fluorescence imaging, Tunable filters, Biological imaging, Imaging systems, Image filtering, Tissues, Preclinical imaging, Light sources and illumination

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SignificancePreclinical fluorescence imaging with NIR-I (700 to 900 nm) illumination and short-wave infrared or NIR-II (1000 to 1700 nm) emission increases tissue penetration depth and improves resolution through decreased scattering. Background autofluorescence decreases signal-to-background ratios (SBR) in fluorescence imaging; maximizing SBR will further improve the impact of deep tissue imaging.AimThe impact of rodent diet, illumination wavelength, and emission range on the background fluorescence and contrast agent SBR were determined to assist with the experimental design of future imaging studies.ApproachFollowing illumination with 670, 760, or 808 nm, autofluorescence in the NIR-I (<975 nm), NIR-II (>1000 nm), and NIR-II LP (>1250 nm) regions was assessed in mice fed chow or a purified diet using an IR VIVO preclinical imager (Photon, Etc.). Comparison of the SBR of liver-localized indocyanine green in the various imaging conditions indicated when gut autofluorescence was a problematic confounder.ResultsMice fed chow exhibit high levels of background autofluorescence in the gastrointestinal tract and, to a lesser extent, skin when illuminated with 670 nm light for NIR-I imaging (700 to 975 nm), interfering with the identification of fluorescently labeled tissue. Background autofluorescence was reduced by more than two orders of magnitude by any of the following changes: (1) purified diet; (2) excitation with 760 or 808 nm illumination; or (3) emission in the NIR-II (1000 to 1600 or 1250 to 1600 nm). Although the SBR was generally sufficient for feature identification except when imaging of chow-fed mice with 670 nm excitation and NIR-I emission, switching to a purified diet, using longer excitation wavelengths, or using longer emission wavelengths improved SBR significantly.ConclusionsSystematic comparison of imaging conditions and diet highlights the reduction in autofluorescence and increase in SBR enabled by intentional choices in the experimental parameters including diet, excitation wavelength, and emission wavelength range.

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Proceedings Article | 17 March 2023 Presentation

PbS/CdS QDs enabled SWIR imaging for lymphatic mapping and multiplexed lymph node imaging (Conference Presentation)

Xingjian Zhong, Amish Patel, Allison Dennis

Proceedings Volume PC12395, PC123950A (2023) https://doi.org/10.1117/12.2649410

KEYWORDS: Lymphatic system, Short wave infrared radiation, Multiplexing, Tissues, Lead, Cadmium sulfide, Visibility, Signal to noise ratio, Semiconductors, Scattering

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Proceedings Article | 9 March 2023 Poster + Paper

Biodegradable and biocompatible semiconductor nanocrystals as NIR-II photoacoustic imaging contrast agents

Vinoin Devpaul Vincely, Swathi Katakam, Kristie Huda, Xingjian Zhong, Joshua Kays, Allison Dennis, Carolyn Bayer

Proceedings Volume 12379, 1237915 (2023) https://doi.org/10.1117/12.2646501

KEYWORDS: Contrast agents, Signal generators, Animals, Semiconductors, Particles, Nanoparticles, Nanocrystals, Image restoration, Photoacoustic imaging, Near infrared

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Proceedings Article | 3 March 2022 Presentation

Oriented conjugation of luciferase to infrared quantum dots enables BRET-based enzyme sensing

Padric Garden, Allison Dennis

Proceedings Volume PC11977, PC1197708 (2022) https://doi.org/10.1117/12.2608887

KEYWORDS: Infrared radiation, Quantum dots, Infrared sensors, Sensors, In vivo imaging, Coating, Bioluminescence, Tissues, Signal generators, Resonance energy transfer

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Proceedings Article | 3 March 2022 Presentation

Engineering PbS/CdS quantum dots for NIR-II multiplexed imaging

Xingjian Zhong, Alexander Saeboe, Umar Iqbal, Dongling Zhang, Maria Moreno, Allison Dennis

Proceedings Volume PC11977, PC1197702 (2022) https://doi.org/10.1117/12.2607967

KEYWORDS: Quantum dots, Multiplexing, Atmospheric particles, Short wave infrared radiation, Quantum efficiency, Infrared radiation, Infrared imaging, In vivo imaging, Signal to noise ratio, Shortwaves

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Proceedings Article | 3 March 2022 Presentation

Generalized targeting and photothermal treatment of infectious agents with biodegradable plasmonic nanocrystals

Joshua Kays, Allison Dennis

Proceedings Volume PC11977, PC1197707 (2022) https://doi.org/10.1117/12.2610100

KEYWORDS: Plasmonics, Nanocrystals, Bacteria, Toxicity, Silver, Resistance, Photothermal effect, Particles, Nanomaterials, Light emitting diodes

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Proceedings Article | 3 March 2022 Presentation

Designing biodegradable inorganic nanoparticles for optical applications in biomedicine

Allison Dennis

Proceedings Volume PC11977, PC119770E (2022) https://doi.org/10.1117/12.2610403

KEYWORDS: Nanoparticles, Copper, Biomedical optics, Toxicity, Particles, Iron, Indium, Copper indium disulfide, Surface plasmons, Semiconductors

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Proceedings Article | 10 March 2020 Presentation

Wide-field dual-probe imaging approach validated using QDs and skin mimicking phantoms (Conference Presentation)

Alexander Saeboe, Keyi Han, Joshua Kays, Reyhaneh Toufanian, Allison Dennis

Proceedings Volume 11254, 112540F (2020) https://doi.org/10.1117/12.2546864

KEYWORDS: Skin, Tumors, Tissues, Imaging systems, Image processing, Semiconductors, Quantum dots, Chemistry, Near infrared, Microscopy

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Proceedings Article | 10 March 2020 Presentation

The toxicity and clearance of copper indium sulfide quantum dots in vivo (Conference Presentation)

Joshua Kays, Alexander Saeboe, Reyhaneh Toufanian, Allison Dennis

Proceedings Volume 11256, 112560B (2020) https://doi.org/10.1117/12.2546747

KEYWORDS: Toxicity, Copper indium disulfide, Copper, Indium, Quantum dots, In vivo imaging, Near infrared, Zinc, Clinical research, Semiconductors

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Proceedings Article | 10 March 2020 Presentation

Development of a biodegradable and non-toxic near infrared optically active quantum dot (Conference Presentation)

Joshua Kays, Allison Dennis

Proceedings Volume 11255, 1125507 (2020) https://doi.org/10.1117/12.2545020

KEYWORDS: Near infrared, Quantum dots, Quantum efficiency, Optical imaging, Biomedical optics, Semiconductors, Cadmium, Lead, Liver, Spleen

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Proceedings Article | 21 February 2020 Paper

Brightness-equalized heavy metal-free quantum dots for biosensing and imaging

Reyhaneh Toufanian, Victoria Kong, Allison Dennis

Proceedings Volume 11255, 112550R (2020) https://doi.org/10.1117/12.2546130

KEYWORDS: Zinc, Heterojunctions, Transmission electron microscopy, Nanocrystals, Absorption, Argon, Quantum dots, Mass attenuation coefficient, Nanoparticles, Sulfur

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Proceedings Article | 22 May 2019 Paper

QD-FRET-based biosensing of small molecule analytes using transcription factor-DNA binding

Thuy Nguyen, Margaret Chern, R. Baer, Andy Fan, Chloe Grazon, James Galagan, Allison Dennis

Proceedings Volume 10892, 108920Q (2019) https://doi.org/10.1117/12.2516576

KEYWORDS: Sensors, Fluorescence resonance energy transfer, Molecules, Proteins, Control systems, Molecular energy transfer, Luminescence, Biological research, Biosensing, Biosensors, Quantum dots, Fluorescence spectroscopy, Fluorescent proteins

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Proceedings Article | 23 February 2018 Presentation + Paper

Pushing indium phosphide quantum dot emission deeper into the near infrared

A. Saeboe, J. Kays, A. Mahler, A. Dennis

Proceedings Volume 10507, 1050715 (2018) https://doi.org/10.1117/12.2295370

KEYWORDS: Quantum dots, Zinc, Indium, Absorbance, Near infrared, Multiplexing, Luminescence, Tumors, Crystals, Tissue optics

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Proceedings Article | 22 February 2017 Presentation + Paper

‘Giant’ nanocrystal quantum dots (gNQDs) as FRET donors

Margaret Chern, Thuy Nguyen, Allison Dennis

Proceedings Volume 10078, 100780L (2017) https://doi.org/10.1117/12.2261716

KEYWORDS: Quantum dots, Nanocrystals, Fluorescence resonance energy transfer, Biosensors, Resonance energy transfer, Molecules, Biological research, Molecular energy transfer, Chemical analysis, Biosensing, Cadmium sulfide, Zinc, Absorption, Sensors, Transmission electron microscopy, Mass attenuation coefficient, Refractive index

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Proceedings Article | 12 February 2011 Paper

Imaging heterostructured quantum dots in cultured cells with epifluorescence and transmission electron microscopy

Erin Rivera, Casilda Trujillo Provencio, Andrea Steinbrueck, Pawan Rastogi, Allison Dennis, Jennifer Hollingsworth, Elba Serrano

Proceedings Volume 7909, 79090N (2011) https://doi.org/10.1117/12.875737

KEYWORDS: Transmission electron microscopy, Quantum dots, Kidney, Cadmium, Zinc, Microscopy, Luminescence, Cancer, Visualization, Signal detection

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Proceedings Article | 18 February 2010 Paper

Quantum dot-fluorescent protein pair as ratiometric pH sensor

Allison Dennis, Gang Bao

Proceedings Volume 7575, 75750C (2010) https://doi.org/10.1117/12.842640

KEYWORDS: Proteins, Fluorescence resonance energy transfer, Sensors, Mass attenuation coefficient, Fluorescent proteins, Absorbance, Luminescence, Quantum dots, Absorption, Adsorption

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Proceedings Article | 13 March 2008 Paper

Förster resonance energy transfer (FRET) between a fluorescent protein and commercially available quantum dots: a comparison

Allison Dennis, Gang Bao

Proceedings Volume 6866, 68660C (2008) https://doi.org/10.1117/12.763727

KEYWORDS: Fluorescence resonance energy transfer, Fluorescent proteins, Quantum dots, Proteins, Quantum efficiency, Luminescence, Molecules, Resonance energy transfer, Zinc, Ions

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