Dr. Anne K. Haudenschild Profile

Dr. Anne K. Haudenschild

at Univ of California Davis

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Publications (9)

SPIE Journal Paper | 16 February 2023 Open Access

Multimodal fluorescence lifetime imaging and optical coherence tomography for longitudinal monitoring of tissue-engineered cartilage maturation in a preclinical implantation model

Xiangnan Zhou, Anne Haudenschild, Cai Li, Laura Marcu

JBO, Vol. 28, Issue 02, 026003, (February 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.2.026003

KEYWORDS: Tissues, Optical coherence tomography, Cartilage, Biological samples, In vivo imaging, Fluorescence, Quantum channels, In vitro testing, Fluorescence lifetime imaging, Nondestructive evaluation

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SignificanceCartilage tissue engineering is a promising strategy for effective curative therapies for treatment of osteoarthritis. However, tissue engineers depend predominantly on time-consuming, expensive, and destructive techniques as quality control to monitor the maturation of engineered cartilage. This practice can be impractical for large-scale biomanufacturing and prevents spatial and temporal monitoring of tissue growth, which is critical for the fabrication of clinically relevant-sized cartilage constructs. Nondestructive multimodal imaging techniques combining fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) hold great potential to address this challenge.AimThe feasibility of using multimodal FLIm–OCT for nondestructive, spatial, and temporal monitoring of self-assembled cartilage tissue maturation in a preclinical mouse model is investigated.ApproachSelf-assembled cartilage constructs were developed for 4 weeks in vitro followed by 4 weeks of in vivo maturation in nude mice. Sterile and nondestructive in situ multispectral FLIm and OCT imaging were carried out at multiple time points (t = 2, 4, and 8 weeks) during tissue development. FLIm and 3D volumetric OCT images were reconstructed and used for the analysis of tissue biochemical homogeneity, morphology, and structural integrity. A biochemical homogeneity index was computed to characterize nonhomogeneous tissue growth at different time points. OCT images were validated against histology.ResultsFLIm detects heterogenous extracellular matrix (ECM) growth of tissue-engineered cartilage. The outer edge of the tissue construct exhibited longer fluorescence lifetime in 375 to 410 and 450 to 485 nm spectral channels, indicating increase in collagen content. Significant (p < 0.05) decrease of construct homogeneity index was observed between t = 2 weeks and t = 4 weeks. Both FLIm and OCT images revealed defects (voids) at the center of the tissue construct during in vitro culture (t = 2 and 4 weeks). Cyst formation during in vivo culture was detected by OCT and confirmed with histology.ConclusionsThe ability of multimodal FLIm–OCT to nondestructively monitor the heterogenous growth of engineered tissue constructs in situ is demonstrated. Spatial and temporal variation of construct ECM component was detected by FLIm. OCT reveals structural defects (voids and cysts). This multimodal approach has great potential to replace costly destructive tests in the manufacturing of tissue-engineered medical products, facilitating their clinical translation.

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Proceedings Article | 11 January 2023 Presentation

Label-free imaging of collagen biomechanics

Benjamin Sherlock, Anne Haudenschild, Xiangnan Zhou, Clay Sheaff, J. Kent Leach, Laura Marcu, Jerry Hu, Kyriacos Athanasiou

Proceedings Volume 12333, 123330C (2023) https://doi.org/10.1117/12.2657927

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Proceedings Article | 9 March 2020 Presentation

FLIm and Raman imaging for detecting micro-environmental changes in bovine pericardium upon genipin cross-linking (Conference Presentation)

Tanveer Ahmed Shaik, Alba Alfonso García, Martin Richter, Florian Korinth, Anne Haudenschild, James Mcmasters, Christoph Krafft, Jürgen Popp, Laura Marcu

Proceedings Volume 11243, 112431B (2020) https://doi.org/10.1117/12.2544225

KEYWORDS: Raman spectroscopy, Fluorescence lifetime imaging, In vitro testing, Analog electronics, Target acquisition, Data acquisition, Luminescence, Tissues, Nondestructive evaluation

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Proceedings Article | 9 March 2020 Presentation

FLIm guided Raman imaging for detecting bovine pericardium cross-links and calcification (Conference Presentation)

Tanveer Ahmed Shaik, Alba Alfonso Garcia, Xiangnan Zhou, Anne Haudenschild, Christoph Krafft, Laura Marcu, Jürgen Popp

Proceedings Volume 11215, 112150I (2020) https://doi.org/10.1117/12.2545632

KEYWORDS: Raman spectroscopy, Fluorescence lifetime imaging, Analog electronics, Target acquisition, Data acquisition, Luminescence, Tissues, Nondestructive evaluation

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Proceedings Article | 7 March 2019 Presentation

Study of vascular tissue grafts cellularization process in bioreactors using label-free fluorescence lifetime imaging (Conference Presentation)

Cai Li, James Mcmasters, Alba Alfonso García, Julien Bec, Anne Haudenschild, Alyssa Panitch, Leigh Griffiths, Laura Marcu

Proceedings Volume 10855, 108550A (2019) https://doi.org/10.1117/12.2510823

KEYWORDS: Tissues, Fluorescence lifetime imaging, Nondestructive evaluation, Control systems, Manufacturing, Multispectral imaging, Stem cells, Prototyping

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Proceedings Article | 7 March 2019 Presentation

Fluorescence lifetime imaging and intravascular ultrasound (FLIm/IVUS) platform for label-free intraluminal characterization of vascular biomaterials in vitro and in vivo (Conference Presentation)

Alba Alfonso García, Anne Haudenschild, James Mcmasters, Julien Bec, Xiangnan Zhou, Alyssa Panitch, Leigh Griffiths, Laura Marcu

Proceedings Volume 10855, 1085509 (2019) https://doi.org/10.1117/12.2510816

KEYWORDS: In vivo imaging, In vitro testing, Fluorescence lifetime imaging, Arteries, Tissues, Intravascular ultrasound, Structured optical fibers, Multispectral imaging, Imaging systems, Natural surfaces

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Proceedings Article | 4 March 2019 Presentation

Multimodal evaluation of tissue engineered cartilage maturation in a pre-clinical implantation model (Conference Presentation)

Anne Haudenschild, Xiangnan Zhou, Cai Li, Jerry Hu, J. Kent Leach, Kyriacos Athanasiou, Laura Marcu

Proceedings Volume 10876, 108760A (2019) https://doi.org/10.1117/12.2509047

KEYWORDS: Tissues, Cartilage, Optical coherence tomography, Nondestructive evaluation, Tissue optics, Optics manufacturing, Imaging systems, Fluorescence lifetime imaging, Fiber optics, Interfaces

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SPIE Journal Paper | 21 December 2018 Open Access

Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging

Xiangnan Zhou, Anne Haudenschild, Benjamin Sherlock, Jerry Hu, J. Kent Leach, Kyriacos Athanasiou, Laura Marcu

JBO, Vol. 23, Issue 12, 126002, (December 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.12.126002

KEYWORDS: Cartilage, Luminescence, Collagen, Fluorescence lifetime imaging, Tissues, Image segmentation, Statistical analysis, Imaging spectroscopy, In vivo imaging, Signal detection

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SPIE Journal Paper | 6 March 2018 Open Access

Nondestructive assessment of collagen hydrogel cross-linking using time-resolved autofluorescence imaging

Benjamin Sherlock, Jenna Harvestine, Debika Mitra, Anne Haudenschild, Jerry Hu, Kyriacos A. Athanasiou, J. Kent Leach, Laura Marcu

JBO, Vol. 23, Issue 03, 036004, (March 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.3.036004

KEYWORDS: Collagen, Luminescence, Nondestructive evaluation, Tissues, Tissue engineering, Statistical analysis, Auto-fluorescence imaging, Imaging systems, Regenerative medicine, Point spread functions

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