Dr. Atsushi Miyawaki Profile

Dr. Atsushi Miyawaki

at RIKEN Ctr. for Brain Science

SPIE Involvement:

Author

Proceedings Article | 13 March 2024 Presentation

Fabrication of 3D CYTOP biochip using two-photon polymerized mold towards super-resolution bioimaging

Mirai Hanzawa, Kotaro Obata, Felix Sima, Hiroyuki Kawano, Kazunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, Koji Sugioka

Proceedings Volume PC12872, PC128720D (2024) https://doi.org/10.1117/12.3001200

KEYWORDS: Fabrication, Super resolution, Polymers, Two photon polymerization, Refractive index, Water, Ultraviolet radiation, Transparency, Resistance, Prostate cancer

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Proceedings Article | 13 March 2024 Presentation

3D microfluidic biochips made of amorphas fluoropolymer CYTOP using two-photon polymerized molding towards super-resolution bioimaging

Kotaro Obata, Mirai Hanzawa, Felix Sima, Hiroyuki Kawano, Kozunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, Koji Sugioka

Proceedings Volume PC12837, PC1283702 (2024) https://doi.org/10.1117/12.3002296

KEYWORDS: Super resolution, Polymers, Microfluidics, 3D microstructuring, Two photon polymerization, Fabrication, Water, Femtosecond phenomena, Transparency, Refractive index

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Proceedings Article | 13 March 2024 Presentation + Paper

Femtosecond laser 3D printing in three colors of fluorescent protein

Daniela Serien, Hiroyuki Kawano, Atsushi Miyawaki, Koji Sugioka, Aiko Narazaki

Proceedings Volume 12837, 1283707 (2024) https://doi.org/10.1117/12.3002034

KEYWORDS: Proteins, Fluorescent proteins, Fabrication, 3D printing, Fluorescence, Light absorption, Femtosecond phenomena, 3D microstructuring, Biomedical applications, Absorption

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Proceedings Article | 2 March 2020 Presentation + Paper

3D glass nanofluidics fabricated by femtosecond laser processing for study of cancer cell metastasis and invasion

F. Sima, H. Kawano, A. Miyawaki, K. Obata, D. Serien, K. Sugioka

Proceedings Volume 11267, 1126714 (2020) https://doi.org/10.1117/12.2547222

KEYWORDS: Glasses, Cancer, Polymers, Microfluidics, Femtosecond phenomena, In vitro testing, Nanolithography, Visualization, Photoresist developing, Laser processing

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Proceedings Article | 2 March 2020 Paper

Recent advances in 3D printing of pure proteinaceous microstructures by femtosecond laser direct write

Daniela Serien, Hiroyuki Kawano, Atsushi Miyawaki, Koji Sugioka

Proceedings Volume 11268, 1126802 (2020) https://doi.org/10.1117/12.2545310

KEYWORDS: Proteins, 3D microstructuring, Molecules, 3D printing, Femtosecond phenomena, Raman spectroscopy, Printing

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Proceedings Article | 12 January 2012 Paper

3D microstructuring inside glass by ultrafast laser

Koji Sugioka, Yasutaka Hanada, Katsumi Midorikawa, Hiroyuki Kawano, Ikuko Ishikawa, Atsushi Miyawaki

Proceedings Volume 8206, 820604 (2012) https://doi.org/10.1117/12.911117

KEYWORDS: Carbon dioxide, Microfluidics, Glasses, 3D microstructuring, Ultrafast lasers, Microorganisms, Optical attenuators, Waveguides, Water, Absorption

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Proceedings Article | 24 December 2011 Paper

Nanoaquarium: integrated microchips fabricated by ultrafast laser for understanding phenomena and functions of microorganisms

Koji Sugioka, Yasutaka Hanada, Katsumi Midorikawa, Hiroyuki Kawano, Ikuko Ishikawa, Atsushi Miyawaki

Proceedings Volume 8204, 820427 (2011) https://doi.org/10.1117/12.904648

KEYWORDS: Carbon dioxide, Microfluidics, Glasses, Microorganisms, Optical attenuators, Water, Waveguides, Femtosecond phenomena, Integration, Microscopes

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Proceedings Article | 18 February 2010 Paper

Nano-aquarium fabrication with cut-off filter for mechanism study of Phormidium assemblage

Y. Hanada, K. Sugioka, I. Ishikawa, H. Kawano, A. Miyawaki, K. Midorikawa

Proceedings Volume 7584, 75840Q (2010) https://doi.org/10.1117/12.845228

KEYWORDS: Optical filters, Glasses, Waveguides, Femtosecond phenomena, Microfluidics, Microscopes, Absorption, Carbon dioxide, Nanolithography, Water

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Proceedings Article | 24 February 2009 Paper

Selective cell culture on UV-transparent polymer by F2 laser modification

Y. Hanada, K. Sugioka, H. Kawano, A. Miyawaki, K. Midorikawa

Proceedings Volume 7201, 72010X (2009) https://doi.org/10.1117/12.808815

KEYWORDS: Pulsed laser operation, Glasses, Polymers, Refractive index, Laser irradiation, Optical microscopes, Phase contrast, Microscopes, Ultraviolet radiation, Absorption

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Proceedings Article | 10 February 2009 Paper

Ultra-high-speed bionanoscope for cell and microbe imaging

T. Goji Etoh, Cuong Vo Le, Hiroyuki Kawano, Ikuko Ishikawa, Atshushi Miyawaki, Vu Dao, Hoang Dung Nguyen, Sayoko Yokoi, Shigeru Yoshida, Hitoshi Nakano, Kohsei Takehara, Yoshiharu Saito

Proceedings Volume 7126, 712605 (2009) https://doi.org/10.1117/12.822466

KEYWORDS: Imaging systems, Cameras, Microscopes, Video, Sensors, Signal detection, Lamps, Charge-coupled devices, Xenon, Luminescence

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Proceedings Article | 23 February 2008 Paper

Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass

Y. Hanada, K. Sugioka, H. Kawano, I. Ishikawa, A. Miyawaki, K. Midorikawa

Proceedings Volume 6880, 68800E (2008) https://doi.org/10.1117/12.762391

KEYWORDS: Glasses, Microorganisms, Femtosecond phenomena, 3D microstructuring, Annealing, Fabrication, Microfluidics, Microscopes, Optical microscopes, Objectives

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Proceedings Article | 12 February 2008 Paper

Microchips fabricated by femtosecond laser micromachining in glass for observation of aquatic microorganisms

Y. Hanada, K. Sugioka, H. Kawano, I. Ishikawa, A. Miyawaki, K. Midorikawa

Proceedings Volume 6886, 68860D (2008) https://doi.org/10.1117/12.762966

KEYWORDS: Glasses, Microorganisms, Femtosecond phenomena, 3D microstructuring, Annealing, Objectives, Microscopes, Optical microscopes, Femtosecond laser micromachining, Wet etching

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Proceedings Article | 13 July 2007 Paper

Ultrafast dynamics in a live cell irradiated by femtosecond laser pulses

Hiroyuki Kawano, Chikako Hara, Takeharu Etoh, Atsushi Miyawaki

Proceedings Volume 6633, 66330J (2007) https://doi.org/10.1117/12.728045

KEYWORDS: Femtosecond phenomena, Plasma, Microscopes, Laser irradiation, Ultrafast phenomena, Video, Objectives, Imaging systems, Refractive index, Water

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Proceedings Article | 14 February 2007 Paper

Cross-talk-free fluorescence cross-correlation spectroscopy by the switching method

Junichi Nishimura, Yasuo Takahashi, Masataka Kinjo, Atsushi Miyawaki

Proceedings Volume 6444, 64440I (2007) https://doi.org/10.1117/12.699992

KEYWORDS: Switching, Signal detection, Proteins, Luminescence, Sensors, Molecular interactions, Cell death, Data acquisition, In vitro testing, Fluorescence spectroscopy

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Proceedings Article | 21 March 2005 Paper

Coherent control of mutiphoton excitation process for biological fluorescence imaging

Katsumi Midorikawa, Jianfan Chen, Hiroyuki Kawano, Yasuo Nabekawa, Akira Suda, Hideaki Mizuno, Atsushi Miyawaki

Proceedings Volume 5714, (2005) https://doi.org/10.1117/12.600034

KEYWORDS: Luminescence, Absorption, Pulsed laser operation, Signal processing, Green fluorescent protein, Femtosecond phenomena, Signal to noise ratio, Phase measurement, Crystals, Molecules

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Proceedings Article | 14 October 2003 Paper

Adaptive control of two-photon fluorescence from green fluorescent protein by shaped femtosecond excitation pulses

Hiroyuki Kawano, Yasuo Nabekawa, Akira Suda, Yu Oishi, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Proceedings Volume 5143, (2003) https://doi.org/10.1117/12.500445

KEYWORDS: Luminescence, Green fluorescent protein, Feedback signals, Femtosecond phenomena, Adaptive control, Phase measurement, Pulsed laser operation, Ocean optics, Beam splitters, Optical filters

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Proceedings Article | 17 September 1999 Paper

Development of an optical coherence tomographic system for imaging of tissue

Jiyuan Liu, Atsushi Miyawaki

Proceedings Volume 3863, (1999) https://doi.org/10.1117/12.364389

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Showing 5 of 17 publications

SPIE Conference Volume | 2 February 2007

Genetically Engineered and Optical Probes for Biomedical Applications IV

Conference Committee Involvement (2)

Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes

23 January 2007 | San Jose, California, United States

Genetically Engineered Probes for Biomedical Applications

24 January 2006 | San Jose, California, United States

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