Ms. Ayumi Komazawa Profile

Ayumi Komazawa

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Proceedings Article | 12 March 2024 Presentation + Paper

Super-resolution Raman probe imaging

Yasuyuki Ozeki, Jingwen Shou, Ayumi Komazawa, Mako Kamiya

Proceedings Volume 12855, 1285503 (2024) https://doi.org/10.1117/12.2691258

KEYWORDS: Biological imaging, Raman spectroscopy, Super resolution, Microscopy, Imaging systems, Diffraction limit

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Super-resolution vibrational microscopy holds great promise for enhancing the multiplexing capabilities of nanometer-scale biological imaging due to the narrower spectral linewidth of molecular vibrations compared to fluorescence. However, current super-resolution vibrational microscopy techniques are plagued by several limitations, such as the requirement for cell fixation, high power consumption, or complex detection setups. Here we describe our recent demonstration of reversible saturable optical Raman transitions (RESORT) microscopy, which addresses these limitations by utilizing photoswitchable stimulated Raman scattering (SRS). To realize RESORT, we developed a bright photoswitchable Raman probe (DAE620). Leveraging the signal depletion capability of DAE620 through a donut-shaped beam, we successfully demonstrate super-resolution vibrational imaging of mammalian cells. This approach provides excellent chemical specificity and achieves spatial resolution beyond the optical diffraction limit. The present results indicate the potential of RESORT microscopy for multiplexed super-resolution imaging of live cells.

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