We introduce and validate a framework for imaging and quantifying active molecule penetration into human skin ex vivo and in vivo. Our approach combines nonlinear imaging microscopy modalities, such as two-photon excited auto-fluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS). The imaging framework is exemplified first on topically applied glycerol diluted in various vehicles such as water and xanthan gel and second, on retinol applied on ex vivo excised human skin. The proposed framework allows for the 3D percutaneous monitoring of active cosmetic molecules and their formulation across human skin. We demonstrate the reservoir property of topically applied xanthan gel vehicle that releases glycerol continuously over time together with the hydrophobic penetration of retinol between corneocyte.
We present a ready to use label free coherent Raman microscope (CARS/SRS) to perform instantaneous virtual histology and chemical imaging for scientific applications. The system is based on a picosecond laser source that can address two vibrational wavenumbers simultaneously across the entire vibrational spectrum (400cm-1 – 4000cm-1). The laser source is coupled to a ready to use beam scanning microscope enabling SRS, CARS and multiphoton microscopy. Targeting the CH2 and CH3 chemical bonds, we demonstrate instantaneous stimulated Raman histology in the brain and the GI. This new, ready to use, SRS/CARS and multiphoton microscope enables SRS detection with exquisite sensitivity (shot noise limited) and ratio-metric measurement by targeting simultaneously two vibrational frequencies that can be tuned anywhere across the vibrational spectrum.
Diagnostic genomic profiling constitutes one of the major challenges to cure brain tumors. The deployment of such analyses depends on the quality of the surgical specimen sent for histopathological examination and further molecular studies. The aim of our study was to assess the potential added value of Stimulated Raman Histology (SRH) for the assessment of freshly excised central nervous system samples. We showed that SRH enabled a near-instant microscopic examination of various central nervous system samples without any tissue processing such as labelling, freezing nor sectioning. Following SRH imaging, we demonstrated that the samples could be readily recovered and reintroduced into a conventional pathology workflow including immunohistochemistry and genomic profiling to establish a definitive diagnosis.
We present a shot noise limited, three-color SRS implementation to address two molecular vibrations simultaneously. The system allows fast, high quality stimulated Raman histology as well as background-free SRS imaging. It is ready to be tested in hospitals for its viability and image quality in comparison to classical rapid histology.
It is based on a mode-locked fs-laser from which 2 narrow-band Stokes laser beams are extracted and subsequently modulated at 13 and 20MHz. The center part of the fs-laser is frequency doubled to pump a picosecond optical parametric oscillator, which can be tuned from 500 to 5000 cm−1.
We introduce and validate a framework for imaging and quantifying active molecule penetration into human skin in vivo. Our approach combines nonlinear imaging microscopy modalities, such as two-photon excited auto-fluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS), together with the use of deuterated active molecules. The imaging framework is exemplified on topically applied glycerol diluted in various vehicles such as water and xanthan gel. In vivo glycerol quantitative percutaneous penetration over time is demonstrated, showing that, contrary to water, xanthan gel vehicle acts as a film reservoir that releases glycerol continuously over time.
We present advances in coherent Raman imaging for gastrointestinal cancer detection. We use stimulated Raman scattering (SRS) combined with second harmonic generation (SHG) to reveal cell nuclei, cytoplasm and collagen simultaneously in human tissues. Cell nuclei, cytoplasm and extra cellular matrix can be visualized in real time with image quality similar to conventional histology.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.