SignificancePeripheral nerves are viscoelastic tissues with unique elastic characteristics. Imaging of peripheral nerve elasticity is important in medicine, particularly in the context of nerve injury and repair. Elasticity imaging techniques provide information about the mechanical properties of peripheral nerves, which can be useful in identifying areas of nerve damage or compression, as well as assessing the success of nerve repair procedures.AimWe aim to assess the feasibility of Brillouin microspectroscopy for peripheral nerve imaging of elasticity, with the ultimate goal of developing a new diagnostic tool for peripheral nerve injury in vivo.ApproachViscoelastic properties of the peripheral nerve were evaluated with Brillouin imaging spectroscopy.ResultsAn external stress exerted on the fixed nerve resulted in a Brillouin shift. Quantification of the shift enabled correlation of the Brillouin parameters with nerve elastic properties.ConclusionsBrillouin microscopy provides sufficient sensitivity to assess viscoelastic properties of peripheral nerves.
Increased permeability in the blood nerve barrier is found after peripheral nerve injury(1) and annexin A-2 has been shown to translocate to Schwann cell surface after injury(2). We hypothesize that this change in permeability can facilitate intraneaural transit of LS-301 to identify nerve injury. We subjected Lewis rats to a mild or severe unilateral sciatic nerve crush injury and performed NIR imaging after administration of Cypate-3 dye and LS-301. Fluorescence within the ROI of the mildly injured nerve and severely injured nerve was significantly increased compared to their contralateral control at 5 hrs after injury. It appears that intravenous administration of near-infrared fluorophores results in detectable changes in nerve fluorescence compared to baseline.
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