Prof. Digant P. Davé Profile

Prof. Digant P. Davé

Professor at Univ of Texas at Arlington

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Author

Publications (18)

Proceedings Article | 19 April 2017 Presentation

Label-free optical detection of action potential in mammalian neurons (Conference Presentation)

Subrata Batabyal, Sarmishtha Satpathy, Loan Bui, Young-Tae Kim, Samarendra Mohanty, Digant Davé

Proceedings Volume 10052, 1005205 (2017) https://doi.org/10.1117/12.2257606

KEYWORDS: Action potentials, Electrophysiology, Singular optics, Neuroscience, Neurons, Optogenetics, Gold, Luminescence, Signal to noise ratio, Phase interferometry

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Electrophysiology techniques are the gold standard in neuroscience for studying functionality of a single neuron to a complex neuronal network. However, electrophysiology techniques are not flawless, they are invasive nature, procedures are cumbersome to implement with limited capability of being used as a high-throughput recording system. Also, long term studies of neuronal functionality with aid of electrophysiology is not feasible. Non-invasive stimulation and detection of neuronal electrical activity has been a long standing goal in neuroscience. Introduction of optogenetics has ushered in the era of non-invasive optical stimulation of neurons, which is revolutionizing neuroscience research. Optical detection of neuronal activity that is comparable to electro-physiology is still elusive. A number of optical techniques have been reported recording of neuronal electrical activity but none is capable of reliably measuring action potential spikes that is comparable to electro-physiology. Optical detection of action potential with voltage sensitive fluorescent reporters are potential alternatives to electrophysiology techniques. The heavily rely on secondary reporters, which are often toxic in nature with background fluorescence, with slow response and low SNR making them far from ideal. The detection of one shot (without averaging)-single action potential in a true label-free way has been elusive so far. In this report, we demonstrate the optical detection of single neuronal spike in a cultured mammalian neuronal network without using any exogenous labels. To the best of our knowledge, this is the first demonstration of label free optical detection of single action potentials in a mammalian neuronal network, which was achieved using a high-speed phase sensitive interferometer. We have carried out stimulation and inhibition of neuronal firing using Glutamate and Tetrodotoxin respectively to demonstrate the different outcome (stimulation and inhibition) revealed in optical signal. We hypothesize that the interrogating optical beam is modulated during neuronal firing by electro-motility driven membrane fluctuation in conjunction with electrical wave propagation in cellular system.

Proceedings Article | 26 April 2016 Presentation

Label free detection of optogenetically stimulated cellular activity by low coherence interferometry (Conference Presentation)

Sarmishtha Satpathy, Subrata Batabyal, Digant Dave, Samarendra Mohanty

Proceedings Volume 9690, 96902D (2016) https://doi.org/10.1117/12.2216404

KEYWORDS: Interferometry, Optogenetics, Luminescence, Refractive index, Coherence (optics), Electrophysiology, System integration, Microscopes, Phase measurement, Spatial resolution

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Proceedings Article | 29 March 2013 Paper

Miniature multifunctional high-performance three-axis positioning and scanning platform

Dragan Avirovik, Digant Dave, Shashank Priya

Proceedings Volume 8690, 869004 (2013) https://doi.org/10.1117/12.2009934

KEYWORDS: Actuators, Lead, Computer aided design, Algorithm development, Optical coherence tomography, Semiconducting wafers, Control systems, Semiconductor lasers, Photomasks, Raster graphics

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Proceedings Article | 30 March 2012 Paper

Miniature image guided three-axis scanning and positioning system

Dragan Avirovik, Digant Dave, Shashank Priya

Proceedings Volume 8343, 83430H (2012) https://doi.org/10.1117/12.917524

KEYWORDS: Lead, Optical coherence tomography, Raster graphics, Computer aided design, Tissue optics, Solid modeling, Surgery, Sensors, Rapid manufacturing, Image quality

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Proceedings Article | 1 March 2010 Paper

Gold nanoprobes for multi-modality tumor imaging

James Nyagilo, Ming Xiao, Xiankai Sun, Digant Davé

Proceedings Volume 7576, 75760X (2010) https://doi.org/10.1117/12.842917

KEYWORDS: Gold, Nanoparticles, Tumors, Nanoprobes, Surface enhanced Raman spectroscopy, Raman spectroscopy, X-ray computed tomography, In vivo imaging, X-rays, Raman scattering

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Proceedings Article | 26 February 2010 Paper

Label-free quantitative detection of biomarkers

Digant Davé, Sajal Chirvi

Proceedings Volume 7568, 75680Z (2010) https://doi.org/10.1117/12.842879

KEYWORDS: Proteins, Sensors, Data acquisition, Interferometry, Cancer, Biological research, Phase interferometry, Interferometers, Interfaces, Reflectivity

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Proceedings Article | 8 January 2007 Paper

A compact optical fiber scanner for medical imaging

Naresh Dhaubanjar, Hans Hu, Digent Dave, Pratibha Phuyal, Jeongsik Sin, Harry Stephanou, J.-C. Chiao

Proceedings Volume 6414, 64141Z (2007) https://doi.org/10.1117/12.695956

KEYWORDS: Magnetism, Scanners, Optical fibers, Nickel, Coating, Fiber optics, Optical coherence tomography, Medical imaging, Sensors, Ferromagnetics

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Proceedings Article | 13 April 2005 Paper

Noncontact subnanometer measurement of transient surface displacement during action potential propagation

Taner Akkin, Digant Dave, H. Grady Rylander, Thomas Milner

Proceedings Volume 5690, (2005) https://doi.org/10.1117/12.592026

KEYWORDS: Nerve, Action potentials, Optical testing, Optical fibers, Glasses, Coherence (optics), Reflection, Reflectometry, Phase modulation, Electrodes

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Proceedings Article | 1 July 2004 Paper

Spatial refractive index measurement of porcine artery using differential phase OCT

Jeehyun Kim, Digant Dave, Chris Rylander, Marc Feldman, Sharon Thomsen, Thomas Milner

Proceedings Volume 5316, (2004) https://doi.org/10.1117/12.529835

KEYWORDS: Refractive index, Arteries, Optical coherence tomography, Tissues, Polarization, Prisms, Tissue optics, Image processing, Phase modulation, Michelson interferometers

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Proceedings Article | 18 June 2004 Paper

Measurement of refractive index variation of physiological analytes using differential-phase OCT

Kirill Larin, Taner Akkin, Thomas Milner, Digand Dave, Massoud Motamedi, Rinat Esenaliev

Proceedings Volume 5325, (2004) https://doi.org/10.1117/12.532004

KEYWORDS: Glucose, Refractive index, Phase shifts, Potassium, Urea, Optical coherence tomography, Tissues, Biological research, Chemical analysis, Optical spheres

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Proceedings Article | 1 July 2003 Paper

Applications of a fiber-based phase-sensitive optical low-coherence reflectometer

Taner Akkin, Digant Dave, Henry Rylander, Thomas Milner

Proceedings Volume 4957, (2003) https://doi.org/10.1117/12.479853

KEYWORDS: Nerve, Polarization, Phase modulation, Reflectometry, Birefringence, Modulators, Coherence (optics), Refractive index, Phase measurement, Microscopes

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Proceedings Article | 1 July 2003 Paper

Low-coherence interferometric detection of photo-thermal and photo-acoustic effects in tissue

Sergey Telenkov, Digant Dave, Thomas Milner

Proceedings Volume 4960, (2003) https://doi.org/10.1117/12.477760

KEYWORDS: Signal detection, Tissue optics, Phase measurement, Interferometers, Photoacoustic spectroscopy, Interferometry, Laser tissue interaction, Pulsed laser operation, Light scattering, Collagen

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Proceedings Article | 27 June 2002 Paper

Polarization-based microscopy using a fiber optic spectral polarimeter

Eunha Kim, Digant Dave, Thomas Milner

Proceedings Volume 4617, (2002) https://doi.org/10.1117/12.472524

KEYWORDS: Polarization, Polarimetry, Fiber optics, Wave plates, Terahertz radiation, Fourier transforms, Phase modulation, Light scattering, Polarizers, Microscopy

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Proceedings Article | 13 June 2002 Paper

Depth-resolved birefringence imaging of the primate retinal nerve fiber layer using polarization-sensitive OCT

Nathaniel Kemp, Jesung Park, Jason Marsack, Digant Dave, Sapun Parekh, Thomas Milner, Henry Rylander

Proceedings Volume 4611, (2002) https://doi.org/10.1117/12.470599

KEYWORDS: Birefringence, Polarization, Optical coherence tomography, Nerve, Optic nerve, Eye, Mirrors, Retina, Head, In vivo imaging

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Proceedings Article | 26 March 2002 Paper

Interferometric fiber-based optical biosensor to measure ultra-small changes in refractive index

Taner Akkin, Digant Dave, Thomas Milner, Henry Rylander

Proceedings Volume 4616, (2002) https://doi.org/10.1117/12.463805

KEYWORDS: Refractive index, Prisms, Phase measurement, Phase modulation, Channel projecting optics, Polarization, Interferometry, Optical biosensors, Optical testing, Tissue optics

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Proceedings Article | 22 May 1997 Paper

Analysis of nonablative skin resurfacing

Thomas Milner, Bahman Anvari, Kurosh Keikhanzadeh, Digant Dave, J. Stuart Nelson, Dennis Goodman, David Hennings, John Baumgardner, Michael Berry

Proceedings Volume 2970, (1997) https://doi.org/10.1117/12.275067

KEYWORDS: Skin, Laser irradiation, Temperature metrology, Injuries, Cryogenics, Wound healing, Proteins, Laser therapeutics, Laser coagulation, Pulsed laser operation

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Proceedings Article | 22 May 1997 Paper

Optical Doppler tomography for noninvasive imaging of in-vivo blood flow

Zhongping Chen, Thomas Milner, Shyam Srinivas, Tore Lindmo, Digant Dave, J. Stuart Nelson

Proceedings Volume 2981, (1997) https://doi.org/10.1117/12.274308

KEYWORDS: Blood circulation, In vivo imaging, Doppler tomography, Tissue optics, Spatial resolution, Blood, Scattering, Velocity measurements, Image resolution, Tissues

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Proceedings Article | 22 May 1997 Paper

Nonablative laser treatment of facial rhytides

Gary Lask, Patrick Lee, Manouchehr Seyfzadeh, J. Stuart Nelson, Thomas Milner, Bahman Anvari, Digant Dave, Roy Geronemus, Leonard Bernstein, Harry Mittelman, Laurie Ridener, Walter Coulson, Bruce Sand, Jon Baumgarder, David Hennings, Richard Menefee, Michael Berry

Proceedings Volume 2970, (1997) https://doi.org/10.1117/12.275064

KEYWORDS: Skin, Laser therapeutics, Collagen, Wound healing, Absorption, Cryogenics, Light scattering, Scattering, Carbon dioxide, Pulsed laser operation

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Showing 5 of 18 publications

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