We present a deep learning-aided imaging system for early detection and classification of live bacterial colonies by capturing time-lapse holographic images of an agar plate and analyzing these images using deep neural networks. We blindly tested our system by identifying Escherichia coli and total coliform bacteria in spiked water samples and successfully detected 90% of the bacterial colonies within 7-10 h, while keeping 99.2~100% precision. We further classified the corresponding species within 7.6-12 h of incubation with 80% accuracy, which represents >12 h time-savings. Our system also achieved a limit-of-detection of ~1 CFU/L within 9 h of total test time.
We present a field-portable and high-throughput imaging flow-cytometer, which performs phenotypic analysis of microalgae using image processing and deep learning. This computational cytometer weighs ~1.6kg, and captures holographic images of water samples containing microalgae, flowing in a microfluidic channel at a rate of 100mL/h. Automated analysis is performed by extracting the spatial and spectral features of the reconstructed images to automatically identify/count the target algae within the sample, using image processing and convolutional neural networks. Changes within the measured features and the composition of the microalgae can be rapidly analyzed to reveal even minute deviations from the normal state of the population.
We present a deep-learning based device to perform automated screening of sickle cell disease (SCD) using images of blood smears captured by a smartphone-based microscope. We experimentally validated the system using 96 blood smears (including 32 positive samples for SCD), each coming from a unique patient. Tested on these blood smears, our framework achieved a 98% accuracy and had an area-under-the-curve (AUC) of 0.998. Since this technique is both low-cost and accurate, it has the potential to improve access to cost-effective screening and monitoring of patients in low resource settings – particularly in areas where existing diagnostic methods are unsuitable.
We report a deep learning-based framework which can be used to screen thin blood smears for sickle-cell-disease using images captured by a smartphone-based microscope. This framework first uses a deep neural network to enhance and standardize the smartphone images to the quality of a diagnostic level benchtop microscope, and a second deep neural network performs cell segmentation. We experimentally demonstrated that this technique can achieve 98% accuracy with an area-under-the-curve (AUC) of 0.998 on a blindly tested dataset made up of thin blood smears coming from 96 patients, of which 32 had been diagnosed with sickle cell disease.
We report a highly-sensitive, high-throughput, and cost-effective bacteria identification system which continuously captures and reconstructs holographic images of an agar-plate and analyzes the time-lapsed images with deep learning models for early detection of colonies. The performance of our system was confirmed by detection and classification of Escherichia coli, Enterobacter aerogenes, and Klebsiella pneumoniae in water samples. We detected 90% of the bacterial colonies and their growth within 7-10h (>95% within 12h) with ~100% precision, and correctly identified the corresponding species within 7.6-12h with 80% accuracy, and achieved time savings of >12h as compared to the gold-standard EPA-approved methods.
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