3D cell culture resembles tissues better than traditional monolayer cultures, which differ greatly from in vivo models. We use this technique to develop multicellular spheroids from two cell lines: MCF-7 (adenocarcinoma) and U87mg (glioblastoma astrocytoma), by forced-floating method. In this work, we research the spheroid behaviour through two optical techniques: photovoltaic tweezers and laser irradiation. We use photovoltaic tweezers to manipulate spheroids and to explore their electric charge. We also investigate their biological response to laser irradiation depending on wavelength and laser power. Finally, cell viability of the spheroids after undergoing each of these optical/optoelectric treatments has been quantified.
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