We have realized video-rate three-photon fluorescence microscopy based on a simple 1320-nm femtosecond source, which is driven by a 24-MHz Cr:forsterite oscillator and fiber-optic nonlinear conversion. The development aims to find a robust pumping source for deep-tissue GFP imaging. We optimized the laser oscillator to deliver 40-nJ output pulse energy at around 1260 nm, and the matching of the laser spectrum and GFP excitation can be realized by precisely control the interplay between self-phase modulation and fiber dispersion. We have obtained clear video-rate three-photon imaging from fluorescent beads, and further optimization leading to deep-tissue GFP imaging will be discussed.
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