We set up the terahertz continuous reflectometry imaging system and the spatial resolution of our system was roughly 0.6×0.6mm at 2.52 THz. We also demonstrated that the paraffin embedded traumatic brain injury (TBI) in rat model sample can be differentiated clearly. The results show that the THz reflection intensity of the TBI area was lower than that of normal area. These promising results suggest that THz reflection imaging has great potential as an alternative method for the fast diagnosis of TBI.
We demonstrated that different degrees of experimental traumatic brain injury (TBI) can be differentiated clearly in fresh slices of rat brain tissues using transmission-type terahertz (THz) imaging system. The high absorption region in THz images corresponded well with the injured area in visible images and magnetic resonance imaging results. The THz image and absorption characteristics of dehydrated paraffin-embedded brain slices and the hematoxylin and eosin (H&E)-stained microscopic images were investigated to account for the intrinsic differences in the THz images for the brain tissues suffered from different degrees of TBI and normal tissue aside from water. The THz absorption coefficients of rat brain tissues showed an increase in the aggravation of brain damage, particularly in the high-frequency range, whereas the cell density decreased as the order of mild, moderate, and severe TBI tissues compared with the normal tissue. Our results indicated that the different degrees of TBI were distinguishable owing to the different water contents and probable hematoma components distribution rather than intrinsic cell intensity. These promising results suggest that THz imaging has great potential as an alternative method for the fast diagnosis of TBI.
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