Test strips have been widely used in various rapid on-site biochemical sensing due to its simple and rapid detections. Unfortunately, many test strips only support qualitative sensing mainly because their detecting results are often determined by visual inspection. In order to solve the problem, we design a handheld fluorescence test strip reader, which can collect fluorescence emission from test strip thus quantify target under detection. The proposed handheld fluorescence test strip reader composes of rather compact optical system including a light emitting diode (LED) for excitation, an optical bandpass filter for fluorescence signal filtering and a smartphone for fluorescence signal collection and data analysis. All the optical elements are integrated using 3-D printed structures and coupled with the smartphone to support on-site detection. In addition, a smartphone application is also designed to automatically quantify fluorescence emission intensity, and it definitely can support quantitative target detection. Proved by a series of applications especially on rapid on-site detections, the handheld fluorescence test strip reader can detect target automatically, rapidly and accurately. Therefore, the proposed handheld fluorescence test strip reader is a potential tool for rapid on-site biochemical detection in various applications including agricultural, biomedical, and environmental fields.
The hydration of biomolecules is closely related to the dynamic process of their functional expression, therefore, characterizing hydration phenomena is a subject of keen interest. However, direct measurements on the global hydration state of biomolecules couldn’t have been acquired using traditional techniques such as thermodynamics, ultrasound, microwave spectroscopy or viscosity, etc. In order to realize global hydration characterization of amino acid such as L-threonine, terahertz time-domain attenuated total reflectance spectroscopy (THz-TDS-ATR) was adopted in this paper. By measuring the complex permittivity of L-threonine solutions with various concentrations in the THz region, the hydration state and its concentration dependence were obtained, indicating that the number of hydrous water decreased with the increase of concentration. The hydration number was evaluated to be 17.8 when the molar concentration of L-threonine was 0.34 mol/L, and dropped to 13.2 when the molar concentration increased to 0.84 mol/L, when global hydration was taken into account. According to the proposed direct measurements, it is believed that the THz-TDS-ATR technique is a powerful tool for studying the picosecond molecular dynamics of amino acid solutions.
Underwater laser imaging is of great significance in underwater search and marine science, etc. However, traditional
underwater laser imaging is often of poor quality with noises and blurs, moreover, the resolution of the image is also
low. In order to obtain clear underwater images with high resolution and quality, here, we have designed a range gated
imaging underwater imaging system and realized an image restoration approach. In this paper, based on the introduction
to the imaging system and image restoration algorithm, the experiment is established by setting the imaging system
under water in the lake to capture the underwater targets. With the proposed underwater image restoration approach,
images of high quality could be retrieved which proves that the method is able to identify the target ~10 meters away
underwater.
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