The laser Raman spectroscopy can provide quick, simple, repeatable, quantitative non-destructive analysis and does not
require sample preparation and is applied in chemistry, physics, biology, medicine and other fields. The
short-wavelength laser (blue or ultraviolet) and the long-wavelength laser (red or infrared) can be used as the excitation
source. In this work, we find that some Raman peak of the molecular characterization will weaken or even disappear
when a longer or shorter wavelength laser used as the excitation source. We present the Raman spectra of benzene and
that of cyclohexane as an example, which are excited by different wavelength laser. The experimental results show that
the Raman peak corresponding to the vibration of CH(CH Raman mode) is much stronger than that of the C Ring(C Ring
Raman mode) when the excitation wavelength is 488 nm. When the wavelength increase to 785 nm, the CH Raman
mode almost disappears and the C Ring Raman mode increases sharply. This experimental phenomenon will be benefits
to the problem-solving of the analysis formula of Raman peak intensity. At the same time, it should be paid attention in
the Raman spectral analysis to the effect of excitation wavelength on it.
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