Dr. Ida Leah L. Gitajn Profile

Dr. Ida Leah L. Gitajn

at Dartmouth-Hitchcock Medical Ctr

SPIE Involvement:

Author

Publications (22)

SPIE Journal Paper | 6 September 2024 Open Access

Open Access

Patient-specific arterial input function for accurate perfusion assessment in intraoperative fluorescence imaging

Yue Tang, Shudong Jiang, Joseph Sottosanti, Thomas Usherwood, Xu Cao, Logan Bateman, Lillian Fisher, Eric Henderson, Ida Leah Gitajn, Jonathan Thomas Elliott

JBO, Vol. 29, Issue S3, S33306, (September 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.S3.S33306

KEYWORDS: Tissues, Bone, Biological imaging, Targeting Task Performance metric, Fluorescence imaging, Skin, Modeling, Accuracy assessment, Blood, Perfusion imaging

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Proceedings Article | 13 March 2024 Presentation

Presentation

Comparison of the effectiveness of two different light spectra in 5-ALA-based antimicrobial photodynamic therapy of orthopaedic implant-relevant biofilms

Logan Bateman, Valentin Demidov, Natalia Demidova, Jason Gunn, Scott Sottosanti, Eric Henderson, Ida Gitajn, Jonathan Elliot

Proceedings Volume PC12822, PC128220G (2024) https://doi.org/10.1117/12.3003128

KEYWORDS: Biofilms, Photodynamic therapy, Light sources, Biomass, Visible radiation, Titanium, Tissues, Surgery, Oxygen, Optical coherence tomography

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Proceedings Article | 13 March 2024 Presentation

Presentation

Evaluating the relationship between microfractures and perfusion during osseointegration with intraoperative dynamic contrast-enhanced fluorescence imaging

Olivia Jackson, Valentin Demidov, Jason Gunn, Samuel Streeter, Jonathan Thomas Elliott, I. Leah Gitajn

Proceedings Volume PC12825, PC128250M (2024) https://doi.org/10.1117/12.3003006

KEYWORDS: Fluorescence imaging, Bone, Perfusion imaging, Biological imaging, Tomography, Surgery, Optical imaging, Ionizing radiation, Interfaces, Indocyanine green

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Proceedings Article | 13 March 2024 Presentation + Paper

Presentation + Paper

Portable red-blue light source for antimicrobial photodynamic therapy of orthopaedic implant biofilms

V. Demidov, J. Sottosanti, N. Demidova, L. Bateman, O. Jackson, P. Craig, D. Hazem, J. Gunn, I. Gitajn, J. Elliott

Proceedings Volume 12822, 1282208 (2024) https://doi.org/10.1117/12.3000416

KEYWORDS: Biofilms, Light sources, Optical coherence tomography, Bioluminescence, Light emitting diodes, Microfluidics, Titanium, Photodynamic therapy, Contamination, Tissues

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Well-organized ecosystems of bacteria colonize orthopaedic devices causing biofilm infections that are notoriously difficult to manage. Biofilms typically exhibit increased resistance to antibiotics leading to treatment failure, and tools for eradicating biofilms that do not increase antibiotic resistance are greatly needed. Antimicrobial photodynamic therapy (aPDT) is a promising form of treatment to combat clinically relevant biofilms. Exogenous provision of 5-aminolevulinic acid (5-ALA) to biofilm-forming clinical strains of E. coli, E. faecalis and S. aureus was recently shown by several research groups to result in the accumulation of sufficient quantities of endogenous photosensitizers porphyrins (protoporphyrin IX, coproporphyrin III and others), via the heme biosynthetic pathway, to produce a significant phototoxic effect when exposed to activating light. For clinical translation of this extremely promising approach, here we develop a portable light source for 5-ALA-based aPDT of orthopaedic implant biofilms, spectrally shaped for optimal porphyrin light absorption at wavelengths range approved by FDA for clinical use. After phantom calibration, we tested it on E.coli-E.faecalis biofilms grown in soft lithography-fabricated microfluidic chips and on methicillin-resistant S. aureus (MRSA) biofilms grown on titanium and stainless steel orthopaedic hardware in custom-designed macrofluidic devices. Successful in-vitro experiments allowed us to conduct a proof-of-concept validation study in a preclinical rat model of MRSA-contaminated open fracture. Following tibia fracture and two hours of wound infection development, a one hour incubation with 20% 5-ALA and treatment with either 90J/cm² or three fractions of 30J/cm² light doses demonstrated 94% and 99% overall reduction of MRSA, respectively, while the temperature of the tissue remained <39°C, below the threshold for thermal damage. The encouraging results suggest further preclinical testing of the developed light source for optimization of aPDT regimen and 5-ALA concentration to reduce the risk of long-term side effects in animal models of contaminated trauma surgery.

Proceedings Article | 13 March 2024 Presentation + Paper

Presentation + Paper

Quantification of methicillin-resistant S. aureus (MRSA) biofilm formation on orthopedic implants using bioluminescence imaging

Olivia Jackson, P. Lucas Craig, Dana Hazem, Natalia Demidova, Jason Gunn, J. Scott Sottosanti, Jonathan Thomas Elliott, Valentin Demidov, I. Leah Gitajn

Proceedings Volume 12822, 1282205 (2024) https://doi.org/10.1117/12.3001766

KEYWORDS: Bioluminescence, Biofilms, Bacteria, Titanium, Stainless steel, Standards development, In vitro testing, Microfluidics, Plating, In vivo imaging

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Proceedings Article | 13 March 2024 Presentation + Paper

Presentation + Paper

Optical coherence tomography-based detection of orthopaedic implant biofilms formed by Methicillin-resistant S.aureus (MRSA)

V. Demidov, N. Demidova, D. Hazem, P. Craig, J. Gunn, O. Jackson, I. Gitajn, I. Vitkin, J. Elliott

Proceedings Volume 12822, 1282206 (2024) https://doi.org/10.1117/12.3001786

KEYWORDS: Biofilms, Optical coherence tomography, Metals, Histograms, Biological research, Bacteria, Scanning electron microscopy, Bioluminescence, Surgery, 3D image processing

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Proceedings Article | 13 March 2024 Poster + Paper

Poster + Paper

Development of a rabbit osseointegration prosthesis model to investigate biofilm infection and antimicrobial photodynamic therapy

Jason Gunn, Valentin Demidov, Olivia Jackson, J. Scott Sottosanti, Logan Bateman, Bailey Fearing, Joseph Hsu, Rachel Seymour, Jonathan Thomas Elliott, I. Leah Gitajn

Proceedings Volume 12822, 128220D (2024) https://doi.org/10.1117/12.3003089

KEYWORDS: Biofilms, Bioluminescence, Animals, Bacteria, Skin, Tissues, Optical coherence tomography, Biological imaging, Surgery, Bone

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In orthopedic trauma surgery, traditional socket-based prostheses are associated with functionally limiting problems affecting 1.7 million amputees in the United States. To improve post-surgical performance and minimize socket-related complications, bone-anchored (osseointegrated) prostheses have been developed. Functionally superior, their widespread implementation has been limited due to infection. In an unacceptable number of patients well-organized biofilm ecosystems of bacteria colonize the osseointegrated implant (OI) and migrate into device-tissue interface, leading to superficial and deep infections, and implant failure. Since the OI implant protrudes through the skin, the site is easily contaminated by microbes. The problem is worsened by increased resistance to antibiotics contributing significantly to surgical outcome failure. Antimicrobial photodynamic therapy (aPDT)—which uses photosensitizers excited with visible light to disrupt biofilms and kill bacteria with produced reactive oxygen species—has been proposed to address this problem. To assess biofilm formation and aPDT effectiveness, we describe a rabbit OI model and steps to investigate the ability of aPDT using 5-Aminolevulinic acid (5-ALA)-based light therapy to control methicillin-resistant S. aureus (MRSA) bacterial infection. As part of an institutionally approved survival surgery, this model involves lower limb amputation at the tibia, OI installation and MRSA inoculation. Within a week of biofilm formation, the optimal aPDT regime of light and 5-ALA dose was applied to the implant-skin interface to eradicate migrating biofilms. We have built a circumferential light source spectrally shaped for optimal photoactivation and cooled without risk of bacteria dispersal. Optical coherence tomography (skin flap healing and side-effects), micro-computed tomography (OI-bone integrity) and bioluminescence (bacterial bioburden before and after aPDT) imaging were used to monitor outcome for up to three weeks post-treatment.

SPIE Journal Paper | 3 January 2024 Open Access

Open Access

Automatic correction of motion artifact in dynamic contrast-enhanced fluorescence imaging during open orthopedic surgery

Yue Tang, Ida Leah Gitajn, Xu Cao, Xinyue Han, Jonathan Elliott, Logan Bateman, Bethany Malskis, Lillian Fisher, Jessica Sin, Eric Henderson, Shudong Jiang

JBO, Vol. 29, Issue 01, 016001, (January 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.1.016001

KEYWORDS: Bone, Fluorescence imaging, Biological imaging, Fluorescence intensity, Surgery, Imaging systems, Tissues, Fluorescence, Histograms, Perfusion imaging

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Proceedings Article | 15 March 2023 Presentation + Paper

Presentation + Paper

Automated motion artifact correction for dynamic contrast-enhanced fluorescence imaging during open orthopedic surgery

Yue Tang, I. Leah Gitajn, Xu Cao, Xinyue Han, Jonathan Elliott, Xiaohan Yu, Logan Bateman, Bethany Malskis, Lillian Fisher, Jessica Sin, Eric Henderson, Brian Pogue, Shudong Jiang

Proceedings Volume 12361, 1236104 (2023) https://doi.org/10.1117/12.2650028

KEYWORDS: Bone, Surgery, Fluorescence imaging, Biological imaging, Perfusion imaging, Targeting Task Performance metric, Indocyanine green, Data acquisition, Orthopedic imaging, Bone imaging

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Proceedings Article | 15 March 2023 Presentation + Paper

Presentation + Paper

Early identification of life-threatening soft-tissue infection using dynamic fluorescence imaging: first-in-kind clinical study of first-pass kinetics

Samuel Streeter, Gabrielle Ray, Logan Bateman, Kendra Hebert, Fallon Bushee, Scott Rodi, I. Leah Gitajn, Jaimo Ahn, Sunil Singhal, Niels Martin, Nicholas Bernthal, Christopher Lee, William Obremskey, Jonathan Shoenecker, Jonathan Thomas Elliott, Eric Henderson

Proceedings Volume 12361, 123610B (2023) https://doi.org/10.1117/12.2648408

KEYWORDS: Tissues, Fluorescence imaging, Surgery, Indocyanine green

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Proceedings Article | 15 March 2023 Presentation + Paper

Presentation + Paper

Fluorescence-guided and molecularly guided debridement: identifying devitalized and infected tissue in orthopaedic trauma

Jonathan Thomas Elliott, Eric Henderson, Samuel Streeter, Valentin Demidov, Xinyue Han, Yue Tang, J. Scott Sottosanti, Logan Bateman, Petr Brůža, Shudong Jiang, I. Leah Gitajn

Proceedings Volume 12361, 1236108 (2023) https://doi.org/10.1117/12.2661243

KEYWORDS: Tissues, Bone, Surgery, Injuries, Imaging systems, Fluorescence, Biofilms, Fluorescence imaging, Fluorescence intensity, Blood circulation

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Proceedings Article | 15 March 2023 Presentation + Paper

Presentation + Paper

Risk prediction on orthopaedic trauma patients for fracture-associated infection using dynamic contrast enhanced-fluorescence imaging

Xinyue Han, Logan Bateman, Paul Werth, Shudong Jiang, I. Leah Gitajn, Jonathan Thomas Elliott

Proceedings Volume 12361, 123610A (2023) https://doi.org/10.1117/12.2650773

KEYWORDS: Surgery, Tissues, Bone, Biological imaging, Machine learning, Feature extraction, Information science, Fluorescence, Cross validation, Visualization

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Proceedings Article | 15 March 2023 Presentation + Paper

Presentation + Paper

Proceduralist criteria for evaluating interface utility of novel imaging modalities in early phase clinical trials: evaluating the need for standardized criteria

Eric Henderson, Jonathan Elliott, Shudong Jiang, I. Leah Gitajn, John Lee, Summer Gibbs, Michael Bouvet, Anita Mahadevan-Jansen, Michael Daly, Samuel Streeter, Keith Paulsen, Brian Pogue, Kimberley Samkoe, Sunil Singhal

Proceedings Volume 12361, 123610F (2023) https://doi.org/10.1117/12.2650756

KEYWORDS: Surgery, Tissues, Target recognition, Standards development, Visualization, Medical imaging, Imaging technologies, Clinical trials, Interfaces, Fluorophores

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Proceedings Article | 14 March 2023 Presentation + Paper

Presentation + Paper

Antimicrobial PDT effectively destroys E. coli and E. faecalis orthopaedic biofilms compared to low efficacy of a tobramycin and vancomycin mixture: an in vitro study using optical coherence tomography

Valentin Demidov, Matthew Bond, I. Leah Gitajn, Carey Nadell, Jonathan Elliott

Proceedings Volume 12358, 1235806 (2023) https://doi.org/10.1117/12.2654683

KEYWORDS: Biofilms, Optical coherence tomography, Vacuum chambers, Photodynamic therapy, Microfluidics, Bacteria, Mixtures, In vitro testing, Absorbance, Visualization

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Proceedings Article | 24 May 2022 Presentation + Paper

Presentation + Paper

Initial experience of perfusion assessment in a rabbit model of orthopaedic trauma surgery using fluorescent microspheres and hyperspectral imaging cryomacrotome

Xinyue Han, Valentin Demidov, Dennis Wirth, Brook Byrd, Scott Davis, Ida Gitajn, Jonathan Elliott

Proceedings Volume 12146, 1214609 (2022) https://doi.org/10.1117/12.2624185

KEYWORDS: Fermium, Bone, Tissues, Surgery, Near infrared, Image processing, Luminescence, Image segmentation, Blood

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Proceedings Article | 4 March 2022 Presentation + Paper

Presentation + Paper

Dynamic contrast-enhanced fluorescence imaging compared with MR imaging in evaluating bone perfusion during open orthopedic surgery

Yue Tang, Jessica Sin, I. Leah Gitajn, Xu Cao, Xinyue Han, Jonathan Elliott, Xiaohan Yu, Melanie Christian, Logan Bateman, Theresa Chockbengboun, Eric Henderson, Brian Pogue, Shudong Jiang

Proceedings Volume 11943, 119430C (2022) https://doi.org/10.1117/12.2608382

KEYWORDS: Magnetic resonance imaging, Bone, Surgery, Software development, Data modeling, Indocyanine green, Orthopedic imaging, Bone imaging

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Proceedings Article | 4 March 2022 Poster + Presentation + Paper

Poster + Presentation + Paper

Validation of dynamic contrast-enhanced bone blood flow imaging technique with fluorescent microspheres

Xinyue Han, Valentin Demidov, Dennis Wirth, Brook Byrd, Scott Davis, Leah Gitajn, Jonathan Elliott

Proceedings Volume 11943, 119430H (2022) https://doi.org/10.1117/12.2608228

KEYWORDS: Fermium, Bone, Blood, Luminescence, Frequency modulation, Blood circulation, Surgery, Tissues, Imaging systems, 3D image processing

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Proceedings Article | 7 December 2021 Paper

Paper

Intraoperative assessment of patient bone viability using texture analysis of dynamic contrast-enhanced fluorescence imaging

Xinyue Han, Valentin Demidov, Shudong Jiang, Ida Leah Gitajn, Jonathan Elliott

Proceedings Volume 11919, 119190A (2021) https://doi.org/10.1117/12.2614457

KEYWORDS: Luminescence, Bone, Tissues, Surgery, Binary data, Image analysis, Image quality, Image classification, Feature extraction, Principal component analysis

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Proceedings Article | 5 March 2021 Paper

Paper

ICG-based dynamic contrast-enhanced fluorescence imaging guided open orthopaedic surgery: pilot patient study

Shudong Jiang, Jonathan Elliott, Jin Xing, Xu Cao, Xiaohan Yu, Xinyue Han, Rebecca Dabrowski, Melanie Christian, Eric Henderson, Brian Pogue, I. Leah Gitajn

Proceedings Volume 11625, 116250W (2021) https://doi.org/10.1117/12.2578976

KEYWORDS: Bone, Luminescence, Tissues, Blood circulation, Surgery, Machine learning, Indocyanine green, Contamination

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SPIE Journal Paper | 31 August 2020 Open Access

Open Access

Perspective on optical imaging for functional assessment in musculoskeletal extremity trauma surgery

Ida Gitajn, Gerard Slobogean, Eric Henderson, Arvind von Keudell, Mitchel Harris, John Scolaro, Nathan O'Hara, Jonathan Elliott, Brian Pogue, Shudong Jiang

JBO, Vol. 25, Issue 08, 080601, (August 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.8.080601

KEYWORDS: Tissues, Bone, Tissue optics, Surgery, Optical imaging, Injuries, Luminescence, Soft tissue optics, Near infrared, Spatial resolution

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Significance: Extremity injury represents the leading cause of trauma hospitalizations among adults under the age of 65 years, and long-term impairments are often substantial. Restoring function depends, in large part, on bone and soft tissue healing. Thus, decisions around treatment strategy are based on assessment of the healing potential of injured bone and/or soft tissue. However, at the present, this assessment is based on subjective clinical clues and/or cadaveric studies without any objective measure. Optical imaging is an ideal method to solve several of these issues. Aim: The aim is to highlight the current challenges in assessing bone and tissue perfusion/viability and the potentially high impact applications for optical imaging in orthopaedic surgery. Approach: The prospective will review the current challenges faced by the orthopaedic surgeon and briefly discuss optical imaging tools that have been published. With this in mind, it will suggest key research areas that could be evolved to help make surgical assessments more objective and quantitative. Results: Orthopaedic surgical procedures should benefit from incorporation of methods to measure functional blood perfusion or tissue metabolism. The types of measurements though can vary in the depth of tissue sampled, with some being quite superficial and others sensing several millimeters into the tissue. Most of these intrasurgical imaging tools represent an ideal way to improve surgical treatment of orthopaedic injuries due to their inherent point-of-care use and their compatibility with real-time management. Conclusion: While there are several optical measurements to directly measure bone function, the choice of tools can determine also the signal strength and depth of sampling. For orthopaedic surgery, real-time data regarding bone and tissue perfusion should lead to more effective patient-specific management of common orthopaedic conditions, requiring deeper penetrance commonly seen with indocyanine green imaging. This will lower morbidity and result in decreased variability associated with how these conditions are managed.

SPIE Journal Paper | 9 June 2020 Open Access

Open Access

Intraoperative fluorescence perfusion assessment should be corrected by a measured subject-specific arterial input function

Jonathan Elliott, Rocco Addante, Gerard Slobogean, Shudong Jiang, Eric Henderson, Brian Pogue, Ida Leah Gitajn

JBO, Vol. 25, Issue 06, 066002, (June 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.6.066002

KEYWORDS: Luminescence, Tissues, Targeting Task Performance metric, Skin, Bone, Brain, Error analysis, Angiography, Surgery, Densitometry

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Proceedings Article | 19 February 2020 Presentation + Paper

Presentation + Paper

Endosteal and periosteal blood flow quantified with dynamic contrast-enhanced fluorescence to guide open orthopaedic surgery

Shudong Jiang, Jonathan Elliott, Jason Gunn, Cao Xu, Alberto Ruiz, Brian Pogue, Eric Henderson, I. Leah Gitajn

Proceedings Volume 11222, 112220F (2020) https://doi.org/10.1117/12.2546173

KEYWORDS: Bone, Luminescence, Blood, Tissues, Blood circulation, Surgery, Imaging systems, Indocyanine green, Video, Injuries

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Showing 5 of 22 publications

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