The luminescence spectra of upconversion nanoparticles (UCNPs) and functionalized UCNPs (UCNPs+folic acid) delivered via subcutaneous and intratumor injection into rats were measured. The particles in normal skin do not diffuse and dissolve, but remain at the introduction site. Inflammatory reaction is developing in the skin when UCNPs were injected. The dense connective tissue capsule is formed around the particles in 3 days after administration. UCNPs were located in the tumor diffusely after 4 daysof injection. Up to 80-90% of the tumor was necrotized after laser irradiation on the fourth day after UCNP administration.
The present work demonstrates the visualization of the intracellular distribution of upconversion nanoparticles (UCNPs) by microscopy with excitation in the NIR spectral range and detection of upconversion luminescence in the VIS range. The cell viability is scored for cytotoxic effects of UCNPs at dark and light exposed conditions. Non-functionalized UCNPs incubated with the cells are found to be endocytosed by cells. The obtained results confirm a high sensitivity of the luminescent UCNPs to the concentration variations within cells. UCNPs are promising alternatives to traditional fluorescent labels for cell imaging and possess prominent potentials in biological and clinical applications.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.