The goal of making calibrated oxygen saturation measurements of blood in retinal arteries and veins via a noninvasive spectroscopic technique has nearly been realized. Semi-continuous advancement in the field of retinal vessel oximetry over the last three decades has resulted in several technologies that seem poised for commercialization. In this paper, we present our instrumentation and technique for making well-calibrated saturation measurements of the blood in retinal vessels. The Eye Oximeter (EOX) is a confocal scanning laser ophthalmoscope capable of acquiring multi-spectral images. Analysis of these spectral vessel images allows spectroscopic determination of the oxygen saturation of blood within each vessel. The primary emphasis of this paper is to illustrate the effect of fundus pigmentation on these oximetric measurements. We show that decreasing fundus reflectivity is mathematically similar to decreasing the vessel thickness. The apparent decreased vessel thickness is a direct consequence of scattering by red blood cells. We present in vitro and in vivo measurements that demonstrate an instrument calibration that is nearly independent of vessel diameter and fundus reflectivity.
To make accurate imagin polarimetry measurements, it is imperative that the individual polarization images comprising a data set are spatially well registered. Both the Stokes Imaging Polarimeter at the University of Alabama in Huntsville and the Multispectral IR Stokes Imagining Polarimeter at Night Vision Laboratories use a rotating quarter-wave linear retarder followed by a fixed linear polarizer to measure Stokes vector images. The rotating retarder cause small amounts of image wander at the detector array. In this paper, we quantify the effects of this image wander on the calculated polarization parameters, and present data indicating the maximum acceptable image wander for typical scenes.
We describe the design and development of an imaging polarimeter that will simultaneously measure Stokes vector images in the mid-wave IR and long-wave IR wavebands. We present an analysis of the expected errors that arise due to spectral variations in the polarization elements of the instrument across each waveband. FInally, instrument calibration and polarization images acquired in the MWIR waveband are presented.
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