Alterations in tissue mechanical properties have emerged as not merely a symptom of cancer but a decisive regulator of various oncogenic processes. The tumor microenvironment exhibits a combination of elastic and viscous traits spanning a wide frequency spectrum, leading to a perceived paradox of both matrix stiffening and ‘liquidization’ that coexist during malignant transformation and metastasis. Yet, seminal mechanobiological studies have largely relied on a single mechanical descriptor of elasticity. We present wideband laser Speckle rHEologicAl micRoscopy (SHEAR) to investigate the elastic and viscous spectral signatures that exist over >5 decades of frequencies in various types of clinical tumor specimens.
The function of orthopedic tissues such as cartilage and bones are particularly susceptible to aberrant mechanical transformation, which has long been associated with changes to the solid tissue components. There is, however, a growing appreciation for the role of interstitial fluid and tissue water content in degenerative mechanical transformation associated with orthopedic diseases. Yet, the contribution of viscous fluid-like behavior to the overall mechanical integrity of orthopedic tissues remains largely unexplored. We demonstrate wideband laser Speckle rHEologicAl micRoscopy (SHEAR) that harnesses speckle fluctuation induced by natural thermal motion of native light scattering tissue structures for microrheological investigation of orthopedic tissues.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.