The demand for widefield OCTA is growing as many retinal diseases exhibit alterations in the peripheral microvascular network at the early stage. In this work, we demonstrate ultrawide-field retinal OCTA over the area spanning 20 mm in diameter in 5 seconds using a 4.74 MHz A-line rate stretched-pulse mode locking (SPML)-OCT system. A concentric-circular scanning with an automatic reference arm length adjustment enabled a wide-field and large-curvature retina imaging within a 2 GHz signal bandwidth. The automatic focal plane adjustment and the deep learning-based denoising were also utilized to enhance vessel visualization in ultrawide-field OCTA images.
Three-dimensional (3D) cellular-resolution imaging of the living human retina over a large field of view would bring a great impact in clinical ophthalmology, potentially finding new biomarkers for early diagnosis and improving the pathophysiological understanding of ocular diseases. While hardware-based and computational Adaptive Optics (AO) Optical Coherence Tomography (OCT) have been developed to achieve cellular-resolution retinal imaging, these approaches support limited 3D imaging fields and their high cost, and their intrinsic hardware complexity limit their practical utility. Here, we demonstrate 3D depth-invariant cellular-resolution imaging of the living human retina over a 3-mm × 3-mm field of view using the intrinsically phase-stable multi-MHz retinal swept-source OCT and tailored computational defocus and aberration correction methods. Single-acquisition imaging of photoreceptor cells, retinal nerve fiber layer, and retinal capillaries is presented across unprecedented imaging fields. By providing wide-field 3D cellular-resolution imaging in the human retina using a standard point-scan architecture routinely used in the clinic, this platform proposes a strategy for expanded utilization of high-resolution retinal imaging in both research and clinical settings.
In vivo computational adaptive optics optical coherence tomography (CAO-OCT) has been challenging due to insufficient volume scan rate. We present video-rate volume-scanning CAO-OCT with a multi-MHz stretched-pulse mode-locked laser.
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