Dr. Juan B. Blanco Canosa Profile

Dr. Juan B. Blanco Canosa

at Scripps Research Institute

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Author

Publications (8)

Proceedings Article | 12 March 2015 Paper

Membrane-targeting peptides for nanoparticle-facilitated cellular imaging and analysis

Joyce Breger, James Delehanty, Kelly Boeneman Gemmill, Lauren Field, Juan Blanco-Canosa, Philip Dawson, Alan Huston, Igor Medintz

Proceedings Volume 9338, 93381P (2015) https://doi.org/10.1117/12.2077026

KEYWORDS: Plasma, Nanoparticles, Luminescence, Analytical research, Quantum dots, Nanomaterials, Metals, Zinc, Biological research, Physiology

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Proceedings Article | 27 August 2014 Paper

Controlling the intracellular fate of nano-bioconjugates: pathways for realizing nanoparticle-mediated theranostics

James Delehanty, Juan Blanco-Canosa, Christopher Bradburne, Kimihiro Susumu, Michael Stewart, Duane Prasuhn, Philip Dawson, Igor Medintz

Proceedings Volume 9166, 91660A (2014) https://doi.org/10.1117/12.2064372

KEYWORDS: Neptunium, Plasma, Nanomaterials, Proteins, Quantum dots, Gold, Nanoparticles, Imaging systems, Toxicity, Analytical research

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Proceedings Article | 2 February 2012 Paper

Further progress in cytosolic cellular delivery of quantum dots

Kelly Boeneman Gemmill, James Delehanty, Michael Stewart, Kimihiro Susumu, Juan Blanco-Canosa, Philip Dawson, Alan Huston, Igor Medintz

Proceedings Volume 8232, 82320B (2012) https://doi.org/10.1117/12.906801

KEYWORDS: Quantum dots, Nanoparticles, Proteins, Biosensing, Toxicity, Luminescence, Polymers, Manufacturing, Zinc, In vivo imaging

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Proceedings Article | 12 February 2011 Paper

Quantum dots as a FRET donor and nanoscaffold for multivalent DNA photonic wires

Kelly Boeneman, Duane Prasuhn, Juan Blanco-Canosa, Philip Dawson, Joseph Melinger, Mario Ancona, Michael Stewart, Kimihiro Susumu, Alan Huston, Igor Medintz

Proceedings Volume 7909, 79090R (2011) https://doi.org/10.1117/12.879272

KEYWORDS: Fluorescence resonance energy transfer, Energy transfer, Quantum efficiency, DNA photonics, Quantum dots, Energy efficiency, Quenching (fluorescence), Composites, Chromophores, Absorption

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Proceedings Article | 5 May 2010 Paper

Quantum dots in life sciences: applications, benefits, and safety issues

James Delehanty, Christopher Bradburne, Kelly Boeneman, Kimihiro Susumu, Bing Mei, Juan Blanco-Canosa, Phillip Dawson, Hedi Mattoussi, Alan Huston, Igor Medintz

Proceedings Volume 7666, 766619 (2010) https://doi.org/10.1117/12.853121

KEYWORDS: Quantum dots, Life sciences, Safety, Semiconductors, Spectroscopy, Quantum efficiency, Polymers, Analytical research, Biology, Optical spectroscopy

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Proceedings Article | 18 February 2010 Paper

Delivery of quantum dot bioconjugates to the cellular cytosol: release from the endolysosomal system

James Delehanty, Christopher Bradburne, Kelly Boeneman, Igor Medintz, Dorothy Farrell, Thomas Pons, Bing Mei, Juan Blanco-Canosa, Philip Dawson, Hedi Mattoussi

Proceedings Volume 7575, 75750S (2010) https://doi.org/10.1117/12.842434

KEYWORDS: Polymers, Quantum dots, Proteins, Semiconductors, Plasma, Analytical research, Toxicity, Tissues, Sodium, Cell biology

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To realize their full potential as intracellular imaging and sensing reagents, robust and efficient methods for the targeted cellular delivery of luminescent semiconductor quantum dots (QDs) must be developed. We have previously shown that QDs decorated with histidine-terminated polyarginine cell-penetrating peptides (CPP) are rapidly and specifically internalized via endocytosis by several mammalian cell lines with no cytotoxicity. Here we demonstrate the long-term intracellular stability and fate of these QD-peptide conjugates. We found that the QD-peptide conjugates remain sequestered within endolysosomal vesicles for up to three days after delivery. However, the CPP appeared to remain stably associated with the QD within these acidic vesicles over this time period. Hence, we explored a number of techniques to either actively deliver QDs directly to the cytosol or to facilitate the endosomal release of endocytosed QDs to the cytosol. Active methods (e.g., electroporation) delivered only modest amounts of QDs to the cytosol that appeared to form aggregates. Delivery of QDs using polymer-based transfection reagents resulted primarily in the endosomal sequestration of the QDs, although one commercial polymer tested delivered QDs to the cytosol but only after several days in culture and with a considerable degree of polymer-induced toxicity. Finally, a modular, amphiphilic peptide containing functionalities designed for cell penetration and vesicular membrane interaction demonstrated the ability to deliver QDs in a well-dispersed manner to the cytosol. This peptide mediated rapid QD uptake followed by a slower efficient endosomal release of the QDs to the cytosol that peaked at 48 hours post-delivery. Importantly, this QD-peptide conjugate elicited minimal cytotoxicity in two cell lines tested. A more detailed understanding of the mechanism of the peptide's uptake and endosomal escape attributes will lead to the design of further QD conjugates for targeted imaging and sensing applications.

Proceedings Article | 3 March 2009 Paper

Using metal complex-labeled peptides for charge transfer-based biosensing with semiconductor quantum dots

Igor Medintz, Thomas Pons, Scott Trammell, Juan Blanco-Canosa, Philip Dawson, Hedi Mattoussi

Proceedings Volume 7189, 71890M (2009) https://doi.org/10.1117/12.808537

KEYWORDS: Metals, Absorption, Ruthenium, Quantum dots, Biosensing, Semiconductors, Nanocrystals, Luminescence, Environmental sensing, Oxidation

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Proceedings Article | 3 March 2009 Paper

Multivalent display of DNA conjugates on semiconductor quantum dots utilizing a novel conjugation method

Duane Prasuhn, Juan Blanco-Canosa, Gary Vora, Hedi Mattoussi, Philip Dawson, Igor Medintz

Proceedings Volume 7189, 71890I (2009) https://doi.org/10.1117/12.808541

KEYWORDS: Quantum dots, Chemistry, Semiconductors, Atmospheric particles, Nanoparticles, Particles, Biomedical optics, Sensors, In vivo imaging, Nanocrystals

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Showing 5 of 8 publications

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