Dr. Junhui Shi Profile

Dr. Junhui Shi

PostDoc at Washington Univ in St Louis

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Author

Publications (24)

SPIE Journal Paper | 30 November 2023 Open Access

Photoacoustic imaging of the dynamics of a dye-labeled IgG4 monoclonal antibody in subcutaneous tissue reveals a transient decrease in murine blood oxygenation under anesthesia

Anjul Khadria, Chad Paavola, Konstantin Maslov, Patricia Brown-Augsburger, Patrick F. Grealish, Emmanuel Lozano, Rui Cao, Junhui Shi, John Beals, Sunday Oladipupo, Lihong Wang

JBO, Vol. 28, Issue 11, 116002, (November 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.11.116002

KEYWORDS: Antibodies, Ear, Blood, Dyes, Photoacoustic imaging, Monoclonal antibodies, Photoacoustic spectroscopy, Tissues, Oxygen, Absorption

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SignificanceOver 100 monoclonal antibodies have been approved by the U.S. Food and Drug Administration (FDA) for clinical use; however, a paucity of knowledge exists regarding the injection site behavior of these formulated therapeutics, particularly the effect of antibody, formulation, and tissue at the injection site. A deeper understanding of antibody behavior at the injection site, especially on blood oxygenation through imaging, will help design improved versions of the therapeutics for a wide range of diseases.AimThe aim of this research is to understand the dynamics of monoclonal antibodies at the injection site as well as how the antibody itself affects the functional characteristics of the injection site [e.g., blood oxygen saturation (sO2)].ApproachWe employed triple-wavelength equipped functional photoacoustic imaging to study the dynamics of dye-labeled and unlabeled monoclonal antibodies at the site of injection in a mouse ear. We injected a near-infrared dye-labeled (and unlabeled) human IgG4 isotype control antibody into the subcutaneous space in mouse ears to analyze the injection site dynamics and quantify molecular movement, as well as its effect on local hemodynamics.ResultsWe performed pharmacokinetic studies of the antibody in different regions of the mouse body to show that dye labeling does not alter the pharmacokinetic characteristics of the antibody and that mouse ear is a viable model for these initial studies. We explored the movement of the antibody in the interstitial space to show that the bolus area grows by ∼300 % over 24 h. We discovered that injection of the antibody transiently reduces the local sO2 levels in mice after prolonged anesthesia without affecting the total hemoglobin content and oxygen extraction fraction.ConclusionsThis finding on local oxygen saturation opens a new avenue of study on the functional effects of monoclonal antibody injections. We also show the suitability of the mouse ear model to study antibody dynamics through high-resolution imaging techniques. We quantified the movement of antibodies at the injection site caused by the interstitial fluid, which could be helpful for designing antibodies with tailored absorption speeds in the future.

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Proceedings Article | 2 June 2021 Presentation + Paper

Mapping human brain function with massively parallel high-speed three-dimensional photoacoustic computed tomography

Shuai Na, Jonathan Russin, Li Lin, Xiaoyun Yuan, Peng Hu, Kay Jann, Lirong Yan, Konstantin Maslov, Junhui Shi, Danny Wang, Charles Liu, Lihong Wang

Proceedings Volume 11642, 116420F (2021) https://doi.org/10.1117/12.2582652

KEYWORDS: Brain, Photoacoustic tomography, Magnetic resonance imaging, Brain mapping, Neuroimaging, Imaging systems, Brain imaging, Oxygen, Neurovascular coupling, Metals

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Proceedings Article | 5 March 2021 Presentation + Paper

Three-dimensional photoacoustic computed tomography for preclinical research and clinical translation

Li Lin, Peng Hu, Xin Tong, Shuai Na, Rui Cao, Xiaoyun Yuan, David Garrett, Junhui Shi, Konstantin Maslov, Lihong Wang

Proceedings Volume 11642, 116420E (2021) https://doi.org/10.1117/12.2582600

KEYWORDS: Photoacoustic tomography, Imaging systems, Pre-clinical research, Heart, Breast, Brain, Hemodynamics, Visualization, Tissues, Spatial resolution

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SPIE Journal Paper | 28 October 2020 Open Access

Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy

Yun He, Junhui Shi, Miguel Pleitez, Konstantin Maslov, Daniel Wagenaar, Lihong Wang

JBO, Vol. 25, Issue 10, 106506, (October 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.10.106506

KEYWORDS: Tissues, Mid-IR, Photoacoustic microscopy, Absorption, Brain, Optical parametric oscillators, Natural surfaces, Ultrasonics, Tissue optics, Spectroscopy

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SPIE Journal Paper | 10 July 2020 Open Access

Photoacoustic topography through an ergodic relay for functional imaging and biometric application in vivo

Yang Li, Lei Li, Liren Zhu, Junhui Shi, Konstantin Maslov, Lihong Wang

JBO, Vol. 25, Issue 07, 070501, (July 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.7.070501

KEYWORDS: Calibration, Photoacoustic spectroscopy, Signal detection, Relays, Oxygen, In vivo imaging, Brain, Photoacoustic tomography, Biometrics

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SPIE Journal Paper | 11 June 2020 Open Access

Wave of single-impulse-stimulated fast initial dip in single vessels of mouse brains imaged by high-speed functional photoacoustic microscopy

Yun He, Junhui Shi, Konstantin Maslov, Rui Cao, Lihong Wang

JBO, Vol. 25, Issue 06, 066501, (June 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.6.066501

KEYWORDS: Brain, Capillaries, Transducers, Raman spectroscopy, Absorption, Ferroelectric polymers, Neuroimaging, Hemodynamics, Microelectromechanical systems, Acoustics

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Proceedings Article | 4 March 2019 Presentation

High-resolution high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy (Conference Presentation)

Junhui Shi, Terence T. Wong, Yun He, Lei Li, Ruiying Zhang, Jeeseong Hwang, Konstantin Maslov, Lihong Wang

Proceedings Volume 10878, 1087825 (2019) https://doi.org/10.1117/12.2515616

KEYWORDS: Mid-IR, Photoacoustic microscopy, Ultraviolet radiation, Tissues, Absorption, Pulsed laser operation, Image resolution, Proteins, Infrared imaging, Imaging spectroscopy

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Proceedings Article | 4 March 2019 Presentation

Multifocal photoacoustic microscopy through an ergodic relay (Conference Presentation)

Yang Li, Terence T. Wong, Junhui Shi, Hsun-Chia Hsu, Lihong Wang

Proceedings Volume 10878, 108781C (2019) https://doi.org/10.1117/12.2513502

KEYWORDS: Imaging systems, Relays, Photoacoustic microscopy, Signal detection, Optical scanning systems, Image resolution, Ultrasonics, Computed tomography, Computing systems, Microlens array

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Proceedings Article | 4 March 2019 Presentation

Dual-wavelength high-speed functional photoacoustic microscopy of mouse brain with a Raman laser at 1-MHz A-line rate (Conference Presentation)

Yun He, Junhui Shi, Konstantin Maslov, Lihong Wang

Proceedings Volume 10878, 1087824 (2019) https://doi.org/10.1117/12.2510658

KEYWORDS: Photoacoustic microscopy, Brain, Raman spectroscopy, Image resolution, Scanners, Transducers, Hemodynamics, Potassium, Gadolinium, Crystals

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Proceedings Article | 27 February 2019 Presentation + Paper

In vivo photoacoustic multi-contrast imaging and detection of protein interactions using a small near-infrared photochromic protein

Lei Li, Anton Shemetov, Peng Hu, Daria Shcherbakova, Junhui Shi, Junjie Yao, Vladislav Verkhusha, Lihong Wang

Proceedings Volume 10878, 1087818 (2019) https://doi.org/10.1117/12.2509198

KEYWORDS: Tumors, Photoacoustic tomography, Proteins, In vivo imaging, Liver, Tissue optics, Tissues, Optical imaging, Near infrared, Photoacoustic spectroscopy

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Proceedings Article | 27 February 2019 Paper

Quantitative cell nuclear imaging by dual-view optical-resolution photoacoustic microscopy

Terence T. Wong, De Cai, Liren Zhu, Junhui Shi, Sung-Liang Chen, Lihong Wang

Proceedings Volume 10878, 108780R (2019) https://doi.org/10.1117/12.2509219

KEYWORDS: Brain, Image resolution, Photoacoustic microscopy, Neuroimaging, Ultraviolet radiation, Tissue optics, Ultrasonics, Nuclear imaging, Tissues, Cancer

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SPIE Journal Paper | 5 December 2018 Open Access

In vivo characterization of connective tissue remodeling using infrared photoacoustic spectra

Yuan Qu, Peng Hu, Junhui Shi, Konstantin Maslov, Peinan Zhao, Chiye Li, Jun Ma, Alejandro Garcia-Uribe, Karen Meyers, Emily Diveley, Stephanie Pizzella, Lisa Muench, Nina Punyamurthy, Naomi Goldstein, Oji Onwumere, Mariana Alisio, Kaytelyn Meyenburg, Jennifer Maynard, Kristi Helm, Emma Altieri, Janessia Slaughter, Sabrina Barber, Tracy Burger, Christine Kramer, Jessica Chubiz, Monica Anderson, Ronald McCarthy, Sarah K. England, George Macones, Molly J. Stout, Methodius Tuuli, Lihong Wang

JBO, Vol. 23, Issue 12, 121621, (December 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.12.121621

KEYWORDS: Scattering, Photoacoustic spectroscopy, Tissue optics, Tissues, Connective tissue, In vivo imaging, Cervix, Infrared photography, Infrared radiation, Monte Carlo methods

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SPIE Journal Paper | 5 December 2018 Open Access

Transvaginal fast-scanning optical-resolution photoacoustic endoscopy

Yuan Qu, Chiye Li, Junhui Shi, Ruimin Chen, Song Xu, Hasan Rafsanjani, Konstantin Maslov, Hannah Krigman, Laura Garvey, Peng Hu, Peinan Zhao, Karen Meyers, Emily Diveley, Stephanie Pizzella, Lisa Muench, Nina Punyamurthy, Naomi Goldstein, Oji Onwumere, Mariana Alisio, Kaytelyn Meyenburg, Jennifer Maynard, Kristi Helm, Janessia Slaughter, Sabrina Barber, Tracy Burger, Christine Kramer, Jessica Chubiz, Monica Anderson, Ronald McCarthy, Sarah England, George Macones, Qifa Zhou, K. Kirk Shung, Jun Zou, Molly Stout, Methodius Tuuli, Lihong Wang

JBO, Vol. 23, Issue 12, 121617, (December 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.12.121617

KEYWORDS: Photoacoustic spectroscopy, Endoscopy, In vivo imaging, Imaging systems, Tissue optics, Microelectromechanical systems, Tissues, Mirrors, Blood vessels, Signal detection

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SPIE Journal Paper | 6 July 2018 Open Access

Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Hsun-Chia Hsu, Lei Li, Junjie Yao, Terence T.W. Wong, Junhui Shi, Ruimin Chen, Qifa Zhou, Lihong Wang

JBO, Vol. 23, Issue 07, 076001, (July 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.7.076001

KEYWORDS: Transducers, Tissue optics, Brain, Image resolution, Ultrasonics, In vivo imaging, Acoustics, Neuroimaging, Signal detection, Photoacoustic microscopy

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Proceedings Article | 22 March 2018 Presentation + Paper

Clinical photoacoustic computed tomography of the human breast in vivo within a single breath hold

Li Lin, Peng Hu, Junhui Shi, Catherine Appleton, Konstantin Maslov, Lihong Wang

Proceedings Volume 10494, 104942X (2018) https://doi.org/10.1117/12.2290987

KEYWORDS: Breast, Photoacoustic tomography, In vivo imaging, Breast cancer, Angiography, Temporal resolution, 3D image reconstruction, Tumors, Elastography, Computing systems

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SPIE Journal Paper | 15 March 2018 Open Access

High-throughput ultraviolet photoacoustic microscopy with multifocal excitation

Toru Imai, Junhui Shi, Terence T. Wong, Lei Li, Liren Zhu, Lihong Wang

JBO, Vol. 23, Issue 03, 036007, (March 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.3.036007

KEYWORDS: Microlens array, Ultrasonics, Transducers, Ultraviolet radiation, Shape memory alloys, Imaging systems, Photoacoustic microscopy, Image resolution, Microlens, Brain

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Proceedings Article | 19 February 2018 Presentation + Paper

Whole-organ atlas imaged by label-free high-resolution photoacoustic microscopy assisted by a microtome

Terence T. Wong, Ruiying Zhang, Hsun-Chia Hsu, Konstantin Maslov, Junhui Shi, Ruimin Chen, K. Kirk Shung, Qifa Zhou, Lihong Wang

Proceedings Volume 10494, 104942N (2018) https://doi.org/10.1117/12.2291056

KEYWORDS: Brain, Neuroimaging, Tissues, Image enhancement, Photoacoustic microscopy, 3D image processing, Ultraviolet radiation, Imaging systems, Tissue optics, 3D modeling

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Proceedings Article | 23 March 2017 Presentation + Paper

Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo

Yun He, Lidai Wang, Junhui Shi, Junjie Yao, Lei Li, Ruiying Zhang, Chih-Hsien Huang, Jun Zou, Lihong Wang

Proceedings Volume 10064, 100641E (2017) https://doi.org/10.1117/12.2255068

KEYWORDS: Laser therapeutics, Melanoma, Imaging systems, In vivo imaging, Photoacoustic microscopy, Skin cancer, Absorption, 3D image processing, Therapeutics, Tumors, Pulsed laser operation, Signal detection, Blood

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Proceedings Article | 23 March 2017 Presentation + Paper

Imaging small animal whole-body dynamics by single-impulse panoramic photoacoustic computed tomography

Lei Li, Liren Zhu, Cheng Ma, Li Lin, Junjie Yao, Lidai Wang, Konstantin Maslov, Ruiying Zhang, Wanyi Chen, Junhui Shi, Lihong Wang

Proceedings Volume 10064, 100640M (2017) https://doi.org/10.1117/12.2251593

KEYWORDS: Photoacoustic tomography, Preclinical imaging, Image resolution, In vivo imaging, Optical imaging, Functional imaging, Molecular imaging, Biology, Pre-clinical research, Visualization, Brain, Neuroimaging, Whole body imaging, Tissue optics, Panoramic photography

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SPIE Journal Paper | 24 October 2016 Open Access

Handheld optical-resolution photoacoustic microscopy

Li Lin, Pengfei Zhang, Song Xu, Junhui Shi, Lei Li, Junjie Yao, Lidai Wang, Jun Zou, Lihong Wang

JBO, Vol. 22, Issue 04, 041002, (October 2016) https://doi.org/10.1117/12.10.1117/1.JBO.22.4.041002

KEYWORDS: Tissue optics, Microelectromechanical systems, Imaging systems, Biomedical optics, Photoacoustic microscopy, Acoustics, Mirrors, Skin, Ear, In vivo imaging

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SPIE Journal Paper | 8 June 2016 Open Access

Grueneisen relaxation photoacoustic microscopy in vivo

Jun Ma, Junhui Shi, Pengfei Hai, Yong Zhou, Lihong Wang

JBO, Vol. 21, Issue 06, 066005, (June 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.6.066005

KEYWORDS: Pulsed laser operation, In vivo imaging, Continuous wave operation, Photoacoustic microscopy, 3D image processing, Brain, Neuroimaging, Image resolution, Biomedical optics, 3D acquisition

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Proceedings Article | 27 April 2016 Presentation

Grueneisen relaxation photoacoustic microscopy in vivo (Conference Presentation)

Jun Ma, Junhui Shi, Pengfei Hai, Yong Zhou, Lihong Wang

Proceedings Volume 9708, 97082Q (2016) https://doi.org/10.1117/12.2211196

KEYWORDS: In vivo imaging, Photoacoustic microscopy, Neuroimaging, Brain, Continuous wave operation, Transducers, Image resolution, Pulsed laser operation, Photoacoustic spectroscopy, Imaging systems

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Proceedings Article | 15 March 2016 Paper

Bessel beam Grueneisen photoacoustic microscopy with extended depth of field

Junhui Shi, Lidai Wang, Cedric Noordam, Lihong Wang

Proceedings Volume 9708, 97083H (2016) https://doi.org/10.1117/12.2211198

KEYWORDS: Photoacoustic microscopy, Bessel beams, Pulsed laser operation, Microscopy, Laser beam diagnostics, Optical resolution, Photoacoustic imaging, Photoacoustic spectroscopy, Focus stacking, Gaussian beams, Continuous wave operation, Nonlinear optics, Carbon, Axicons, Image resolution

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SPIE Journal Paper | 2 November 2015 Open Access

Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field

Junhui Shi, Lidai Wang, Cedric Noordam, Lihong Wang

JBO, Vol. 20, Issue 11, 116002, (November 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.11.116002

KEYWORDS: Pulsed laser operation, Bessel beams, Nonlinear optics, Continuous wave operation, Laser beam diagnostics, Carbon, Photoacoustic microscopy, Image resolution, Axicons, Focus stacking

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