Ultraviolet C (UVC) radiation is an effective method of disinfection in public areas. As an emerging source of UVC, ultraviolet C light-emitting diodes(UVC-LEDs) are practical for the benefits of mercury-free nature, long life span and multiple applications. However, it takes a longer disinfection time using UVC-LEDs result from the low output light power of UVC-LEDs. In this study, a pulsed light (PL) radiated by UVC-LED with increased instantaneous voltage and current was applied for the irradiation of Escherichia coli (E. coli). It demonstrated a nonlinear relationship between the instantaneous power of UVC light and the sterilization effect of E. coli. In addition, the log-inactivation of E. coli irradiated by PL from 10 microseconds at 1kHz during 60 seconds was compared with those irradiated by continuous light (CW) during the same exposed time. The result shown the sterilization of E. coli in PL was more effective than CW during same time.
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