Colonoscopy is the most widely prescribed colonic imaging method for the clinical surveillance of colorectal cancer for the detection of adenomatous polyps. However, some adenomas appear diminutive and flat, making them difficult to discriminate from non-neoplastic tissue even for the most experienced colonoscopists. We report here an optical system for performing excitation resolved visible and near-infrared autofluorescence imaging and hyperspectral imaging for the accurate distinction between neoplastic and non-neoplastic tissue. This is demonstrated on ex vivo colorectal tissues using our multimodal imaging system, which has potential applications for the in vivo endoscopic imaging and diagnosis of colorectal cancer tissue.
This paper presents an optical system for performing excitation resolved imaging of fluorescent
dyes, tissue phantoms and ex vivo tissues. The excitation source was a supercontinuum generated in
a highly nonlinear fibre, spectrally filtered using dispersive optics and a movable slit or digital
micromirror device. This allowed excitation with multiple spectra, which may be chosen to
optimize the fluorescence yield from a particular fluorophore, or to maximize discrimination
between multiple labels. As an initial validation, the analysis of fluorescent dye solutions
embedded in collagen gels in the presence of scatterers and absorbers by using fixed reference
isomap (FR-IsoMap) is presented, followed by initial evaluation with human lung bronchial tissue.
The proposed system has potential applications for in vivo fluorescence endoscopy of cancerous
tissues through excitation spectral selection and analysis with FR-IsoMap.
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