We present a non-invasive (label-free) and 3-D method for anti-cancer drug response evaluation of human originated tumor spheroids. Our method is a combination of an OCT microscope and a statistical analysis framework of the OCT signal temporal fluctuations induced by the intra-cellular motions. This method allows the visualization of the 3-D metabolic activity of tumor spheroids and its alteration induced by the drug applications. Two human originated tumor spheroid’s drug responses were evaluated including breast adenocarcinoma (MCF7 cell-line) and colorectal carcinoma (HT-29 cell-line). The drug response was not only visualized as an images, but also quantitative analysis has been performed.
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